GRecon: A Method for the Lipid Reconstitution of Membrane Proteins

Density gradients with increasing concentrations of cyclodextrin and detergent‐destabilized liposomes are loaded with detergent‐solubilized membrane proteins in a new approach for reconstituting membrane proteins. During centrifugation, the detergent is gradually replaced by the lipid. This gentle m...

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Veröffentlicht in:	Angewandte Chemie International Edition 2012-08, Vol.51 (33), p.8343-8347
Hauptverfasser:	Althoff, Thorsten, Davies, Karen M., Schulze, Sabrina, Joos, Friederike, Kühlbrandt, Werner
Format:	Artikel
Sprache:	eng
Schlagworte:	Centrifugation, Density Gradient - instrumentation Centrifugation, Density Gradient - methods Communications Concentration gradient Cyclodextrins Density gradients Detergents Detergents - chemistry Lipids Lipids - chemistry Liposomes membrane proteins Membrane Proteins - chemistry Membranes Proteins structural biology
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Beschreibung
Zusammenfassung:	Density gradients with increasing concentrations of cyclodextrin and detergent‐destabilized liposomes are loaded with detergent‐solubilized membrane proteins in a new approach for reconstituting membrane proteins. During centrifugation, the detergent is gradually replaced by the lipid. This gentle method separates proteoliposomes from empty liposomes and is particularly suitable for the lipid reconstitution of large, fragile membrane proteins.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.201202094