1‑Methylthio‑d‑xylulose 5‑Phosphate Methylsulfurylase: A Novel Route to 1‑Deoxy‑d‑xylulose 5‑Phosphate in Rhodospirillum rubrum
Rhodospirillum rubrum produces 5-methylthioadenosine (MTA) from S-adenosylmethionine in polyamine biosynthesis; however, R. rubrum lacks the classical methionine salvage pathway. Instead, MTA is converted to 5-methylthio-d-ribose 1-phosphate (MTR 1-P) and adenine; MTR 1-P is isomerized to 1-methylth...
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Veröffentlicht in: | Biochemistry (Easton) 2012-10, Vol.51 (42), p.8324-8326 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Rhodospirillum rubrum produces 5-methylthioadenosine (MTA) from S-adenosylmethionine in polyamine biosynthesis; however, R. rubrum lacks the classical methionine salvage pathway. Instead, MTA is converted to 5-methylthio-d-ribose 1-phosphate (MTR 1-P) and adenine; MTR 1-P is isomerized to 1-methylthio-d-xylulose 5-phosphate (MTXu 5-P) and reductively dethiomethylated to 1-deoxy-d-xylulose 5-phosphate (DXP), an intermediate in the nonmevalonate isoprenoid pathway [Erb, T. J., et al. (2012) Nat. Chem. Biol., in press]. Dethiomethylation, a novel route to DXP, is catalyzed by MTXu 5-P methylsulfurylase. An active site Cys displaces the enolate of DXP from MTXu 5-P, generating a methyl disulfide intermediate. |
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ISSN: | 0006-2960 1520-4995 |
DOI: | 10.1021/bi301215g |