Convergent Chemical Synthesis of [Lysine24, 38, 83] Human Erythropoietin

A new route to EPO: Nonglycosylated human erythropoietin (EPO) was prepared using a convergent chemical ligation synthetic strategy. The synthetic [Lysine24, 38, 83] EPO analogue, which was purified by HPLC and has the correct molecular weight (see picture), shows well‐defined covalent structure, is...

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Veröffentlicht in:	Angewandte Chemie International Edition 2012-01, Vol.51 (4), p.993-999
Hauptverfasser:	Liu, Suhuai, Pentelute, Brad L., Kent, Stephen B. H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Substitution Cell Line Circular Dichroism erythropoietin Erythropoietin - analogs & derivatives Erythropoietin - chemical synthesis Erythropoietin - metabolism Humans kinetically controlled ligation native chemical ligation Peptides - chemical synthesis Peptides - chemistry Protein Folding proteins solid-phase synthesis Solid-Phase Synthesis Techniques
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Beschreibung
Zusammenfassung:	A new route to EPO: Nonglycosylated human erythropoietin (EPO) was prepared using a convergent chemical ligation synthetic strategy. The synthetic [Lysine24, 38, 83] EPO analogue, which was purified by HPLC and has the correct molecular weight (see picture), shows well‐defined covalent structure, is correctly folded, and is biologically active in a factor‐dependent cell line assay.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.201106060