Association of a functional polymorphism (Gln261Arg) in 12-lipoxygenase with breast cancer
The overexpression of arachidonyl lipoxygenase-12 (ALOX12) in breast cancer has been reported. Hence, we examined whether a non-synonymous polymorphism of ALOX12 (mRNA, A835G; Gln261Arg) is associated with breast cancer in females. The polymorphism was detected in genomic DNA by PCR-RFLP. The associ...
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| Veröffentlicht in: | Experimental and therapeutic medicine 2011-03, Vol.2 (2), p.317-323 |
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| creator | PRASAD, VIDUDALA V.T.S KOLLI, PADMA MOGANTI, DIVYA |
| description | The overexpression of arachidonyl lipoxygenase-12 (ALOX12) in breast cancer has been reported. Hence, we examined whether a non-synonymous polymorphism of ALOX12 (mRNA, A835G; Gln261Arg) is associated with breast cancer in females. The polymorphism was detected in genomic DNA by PCR-RFLP. The association between the A835G polymorphism and breast cancer risk was measured by odds ratio (OR) with 95% confidence intervals (CIs) using Fisher's exact test, and differences were considered significant at p |
| doi_str_mv | 10.3892/etm.2011.209 |
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Hence, we examined whether a non-synonymous polymorphism of ALOX12 (mRNA, A835G; Gln261Arg) is associated with breast cancer in females. The polymorphism was detected in genomic DNA by PCR-RFLP. The association between the A835G polymorphism and breast cancer risk was measured by odds ratio (OR) with 95% confidence intervals (CIs) using Fisher's exact test, and differences were considered significant at p<0.05. The frequencies of AA (wild-type), GG (homozygous variant) and AG (heterozygous variant) were 59.5, 0.9 and 39.6% in the controls, and 39.3, 2.5 and 58.2% in the breast cancer cases, respectively. The frequency of the AG genotype was higher in the patients compared to the controls (p<0.0014). The frequency of the GG variant was 2.5 and 0.9% in the cancer subjects and controls, respectively. The relative risk of breast cancer was 2 times greater (OR=2.227) at 95% CI when compared to the relative risk of the heterozygous variant. For the GG genotype, the risk was 4 times greater (OR=4.125) at 95% CI than that of the controls, suggesting a positive association of the AG genotype with the occurrence of breast cancer. The frequencies of the polymorphism were different in different populations. The Arg/Gln and Arg/Arg variants were associated with an increased risk of breast cancer, and the frequencies of the variants differed considerably among various populations. The identification of a gene with links to breast cancer may impact screening, diagnosis and drug development.</description><identifier>ISSN: 1792-0981</identifier><identifier>EISSN: 1792-1015</identifier><identifier>DOI: 10.3892/etm.2011.209</identifier><identifier>PMID: 22977504</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>12-lipoxygenase ; 2-hydroxyeicosatetraenoic acid ; arachidonyl lipoxygenase-12 ; breast cancer ; lipoxygenase ; polymorphism and gene variants ; single-nucleotide polymorphism</subject><ispartof>Experimental and therapeutic medicine, 2011-03, Vol.2 (2), p.317-323</ispartof><rights>Copyright © 2011, Spandidos Publications</rights><rights>Copyright © 2011, Spandidos Publications 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-20255254a96d6c0484e9fb41ee26483f7137448bbcfbad168ee0651fc72e0813</citedby><cites>FETCH-LOGICAL-c416t-20255254a96d6c0484e9fb41ee26483f7137448bbcfbad168ee0651fc72e0813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3440668/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3440668/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22977504$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PRASAD, VIDUDALA V.T.S</creatorcontrib><creatorcontrib>KOLLI, PADMA</creatorcontrib><creatorcontrib>MOGANTI, DIVYA</creatorcontrib><title>Association of a functional polymorphism (Gln261Arg) in 12-lipoxygenase with breast cancer</title><title>Experimental and therapeutic medicine</title><addtitle>Exp Ther Med</addtitle><description>The overexpression of arachidonyl lipoxygenase-12 (ALOX12) in breast cancer has been reported. Hence, we examined whether a non-synonymous polymorphism of ALOX12 (mRNA, A835G; Gln261Arg) is associated with breast cancer in females. The polymorphism was detected in genomic DNA by PCR-RFLP. The association between the A835G polymorphism and breast cancer risk was measured by odds ratio (OR) with 95% confidence intervals (CIs) using Fisher's exact test, and differences were considered significant at p<0.05. The frequencies of AA (wild-type), GG (homozygous variant) and AG (heterozygous variant) were 59.5, 0.9 and 39.6% in the controls, and 39.3, 2.5 and 58.2% in the breast cancer cases, respectively. The frequency of the AG genotype was higher in the patients compared to the controls (p<0.0014). The frequency of the GG variant was 2.5 and 0.9% in the cancer subjects and controls, respectively. The relative risk of breast cancer was 2 times greater (OR=2.227) at 95% CI when compared to the relative risk of the heterozygous variant. For the GG genotype, the risk was 4 times greater (OR=4.125) at 95% CI than that of the controls, suggesting a positive association of the AG genotype with the occurrence of breast cancer. The frequencies of the polymorphism were different in different populations. The Arg/Gln and Arg/Arg variants were associated with an increased risk of breast cancer, and the frequencies of the variants differed considerably among various populations. The identification of a gene with links to breast cancer may impact screening, diagnosis and drug development.</description><subject>12-lipoxygenase</subject><subject>2-hydroxyeicosatetraenoic acid</subject><subject>arachidonyl lipoxygenase-12</subject><subject>breast cancer</subject><subject>lipoxygenase</subject><subject>polymorphism and gene variants</subject><subject>single-nucleotide polymorphism</subject><issn>1792-0981</issn><issn>1792-1015</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNpVkd9rFDEQx4NYbKl981nyZgW3zWTz80U4ilah0Jc-9SVks9m7yG6yJnvq_ffmvPPQIcwkzIfvDPki9AbITas0vfXLdEMJQE36BboAqWkDBPjL451oBefoqpRvpAYXoBR_hc4p1VJywi7Q86qU5IJdQoo4DdjiYRvd_mVHPKdxN6U8b0KZ8PX9GKmAVV6_xyFioM0Y5vRrt_bRFo9_hmWDu-xtWbCz0fn8Gp0Ndiz-6lgv0dPnT093X5qHx_uvd6uHxjEQS0MJ5ZxyZrXohSNMMa-HjoH3VDDVDhJayZjqOjd0tgehvCeCw-Ak9URBe4k-HmTnbTf53vm4ZDuaOYfJ5p1JNpj_OzFszDr9MC1jRAhVBa6PAjl93_qymCkU58fRRp-2xYDiWkgJnFf0wwF1OZWS_XAaA8TsDTHVELM3pCZd8bf_rnaC_35_Bd4dgDLb2Ic-lRNThRpC_5wWZPsbnv-TuA</recordid><startdate>20110301</startdate><enddate>20110301</enddate><creator>PRASAD, VIDUDALA V.T.S</creator><creator>KOLLI, PADMA</creator><creator>MOGANTI, DIVYA</creator><general>D.A. Spandidos</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20110301</creationdate><title>Association of a functional polymorphism (Gln261Arg) in 12-lipoxygenase with breast cancer</title><author>PRASAD, VIDUDALA V.T.S ; KOLLI, PADMA ; MOGANTI, DIVYA</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-20255254a96d6c0484e9fb41ee26483f7137448bbcfbad168ee0651fc72e0813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>12-lipoxygenase</topic><topic>2-hydroxyeicosatetraenoic acid</topic><topic>arachidonyl lipoxygenase-12</topic><topic>breast cancer</topic><topic>lipoxygenase</topic><topic>polymorphism and gene variants</topic><topic>single-nucleotide polymorphism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PRASAD, VIDUDALA V.T.S</creatorcontrib><creatorcontrib>KOLLI, PADMA</creatorcontrib><creatorcontrib>MOGANTI, DIVYA</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Experimental and therapeutic medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PRASAD, VIDUDALA V.T.S</au><au>KOLLI, PADMA</au><au>MOGANTI, DIVYA</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Association of a functional polymorphism (Gln261Arg) in 12-lipoxygenase with breast cancer</atitle><jtitle>Experimental and therapeutic medicine</jtitle><addtitle>Exp Ther Med</addtitle><date>2011-03-01</date><risdate>2011</risdate><volume>2</volume><issue>2</issue><spage>317</spage><epage>323</epage><pages>317-323</pages><issn>1792-0981</issn><eissn>1792-1015</eissn><abstract>The overexpression of arachidonyl lipoxygenase-12 (ALOX12) in breast cancer has been reported. Hence, we examined whether a non-synonymous polymorphism of ALOX12 (mRNA, A835G; Gln261Arg) is associated with breast cancer in females. The polymorphism was detected in genomic DNA by PCR-RFLP. The association between the A835G polymorphism and breast cancer risk was measured by odds ratio (OR) with 95% confidence intervals (CIs) using Fisher's exact test, and differences were considered significant at p<0.05. The frequencies of AA (wild-type), GG (homozygous variant) and AG (heterozygous variant) were 59.5, 0.9 and 39.6% in the controls, and 39.3, 2.5 and 58.2% in the breast cancer cases, respectively. The frequency of the AG genotype was higher in the patients compared to the controls (p<0.0014). The frequency of the GG variant was 2.5 and 0.9% in the cancer subjects and controls, respectively. The relative risk of breast cancer was 2 times greater (OR=2.227) at 95% CI when compared to the relative risk of the heterozygous variant. For the GG genotype, the risk was 4 times greater (OR=4.125) at 95% CI than that of the controls, suggesting a positive association of the AG genotype with the occurrence of breast cancer. The frequencies of the polymorphism were different in different populations. The Arg/Gln and Arg/Arg variants were associated with an increased risk of breast cancer, and the frequencies of the variants differed considerably among various populations. The identification of a gene with links to breast cancer may impact screening, diagnosis and drug development.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>22977504</pmid><doi>10.3892/etm.2011.209</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 12-lipoxygenase 2-hydroxyeicosatetraenoic acid arachidonyl lipoxygenase-12 breast cancer lipoxygenase polymorphism and gene variants single-nucleotide polymorphism |
| title | Association of a functional polymorphism (Gln261Arg) in 12-lipoxygenase with breast cancer |
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