Cyclic Caged Morpholinos: Conformationally Gated Probes of Embryonic Gene Function

Feeling a bit cagey: Morpholino‐based antisense reagents have been caged through oligonucleotide cyclization (see scheme), enabling photocontrol of gene expression in zebrafish embryos and larvae. Using these reagents, the timing of exocrine cell fate commitment in the developing pancreas has been e...

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Veröffentlicht in:	Angewandte Chemie International Edition 2012-07, Vol.51 (28), p.6908-6911
Hauptverfasser:	Yamazoe, Sayumi, Shestopalov, Ilya A., Provost, Elayne, Leach, Steven D., Chen, James K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals antisense oligonucleotides Cell Differentiation Danio rerio developmental biology Embryo, Nonmammalian - cytology Embryo, Nonmammalian - metabolism Embryos Gene Expression Gene Expression Regulation, Developmental Genes Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Larva - cytology Larva - metabolism Larvae Morpholinos - pharmacology Oligonucleotides Oligonucleotides - pharmacology Organogenesis Pancreas Pancreas - embryology Pancreas - metabolism Time measurements Zebrafish Zebrafish - embryology Zebrafish - genetics Zebrafish - metabolism
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Beschreibung
Zusammenfassung:	Feeling a bit cagey: Morpholino‐based antisense reagents have been caged through oligonucleotide cyclization (see scheme), enabling photocontrol of gene expression in zebrafish embryos and larvae. Using these reagents, the timing of exocrine cell fate commitment in the developing pancreas has been examined.
ISSN:	1433-7851 1521-3773
DOI:	10.1002/anie.201201690