A restriction endonuclease from Staphylococcus aureus

A restriction endonuclease from Staphylococcus aureus

A specific endonuclease, Sau 3AI, has been partially purified from Staphylococcus aureus strain 3A by DEAE-cellulose chromatography. The enzyme cleaves adenovirus type 5 DNA many times, SV40 DNA eight times but does not cleave double-stranded phi X174 DNA. It recognizes the sequence (see article) an...

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Veröffentlicht in:Nucleic acids research 1976-11, Vol.3 (11), p.3193-3202
Hauptverfasser: Sussenbach, J S, Monfoort, C H, Schiphof, R, Stobberingh, E E
Format: Artikel
Sprache:eng
Schlagworte:
Base Sequence
DNA
DNA Restriction Enzymes - isolation & purification
DNA Restriction Enzymes - metabolism
Endonucleases - metabolism
Molecular Weight
Staphylococcus aureus
Staphylococcus aureus - enzymology
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Beschreibung
Zusammenfassung:A specific endonuclease, Sau 3AI, has been partially purified from Staphylococcus aureus strain 3A by DEAE-cellulose chromatography. The enzyme cleaves adenovirus type 5 DNA many times, SV40 DNA eight times but does not cleave double-stranded phi X174 DNA. It recognizes the sequence (see article) and cleaves as indicated by the arrows. Evidence is presented that this enzyme plays a role in the biological restriction-modification system of Staphylococcus aureus strain 3A.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/3.11.3193