Segmental Isotopic Labeling of Ubiquitin Chains To Unravel Monomer-Specific Molecular Behavior

Segmental Isotopic Labeling of Ubiquitin Chains To Unravel Monomer-Specific Molecular Behavior

“Seeing” each monomer in ubiquitin chains: By combination of the chemical synthesis of one ubiquitin monomer with recombinant expression of its 15N‐containing counterpart, the labeling of ubiquitins in non‐enzymatically assembled di‐ubiquitin chains was achieved, to enable monomer‐specific studies i...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Angewandte Chemie International Edition 2011-11, Vol.50 (47), p.11210-11214
Hauptverfasser: Castañeda, Carlos A., Spasser, Liat, Bavikar, Sudhir N., Brik, Ashraf, Fushman, David
Format: Artikel
Sprache:eng
Schlagworte:
chain structures
DNA Repair Enzymes - chemistry
DNA-Binding Proteins - chemistry
Isotope Labeling
isotopic labeling
Ligands
Lysine - chemistry
Models, Molecular
Nitrogen Isotopes
Nuclear Magnetic Resonance, Biomolecular
Polyubiquitin - chemical synthesis
Polyubiquitin - chemistry
Protein Conformation
protein structures
Protein Transport
semisynthesis
ubiquitin
Ubiquitin - chemical synthesis
Ubiquitin - chemistry
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:“Seeing” each monomer in ubiquitin chains: By combination of the chemical synthesis of one ubiquitin monomer with recombinant expression of its 15N‐containing counterpart, the labeling of ubiquitins in non‐enzymatically assembled di‐ubiquitin chains was achieved, to enable monomer‐specific studies in solution. This allowed the first characterization of the structure, dynamics, intrachain interactions, and ligand binding of Lys33‐linked di‐ubiquitin.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201104649