Sensitive and High-Throughput Isolation of Rare Cells from Peripheral Blood with Ensemble-Decision Aliquot Ranking

Enrichment of cells: An approach called ensemble‐decision aliquot ranking (eDAR) for isolating rare cells from peripheral blood is described. The eDAR process has a recovery of over 93 % (number of runs n=9) with a zero false positive rate (n=8) and provides direct easy access to individual isolated...

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Veröffentlicht in:	Angewandte Chemie International Edition 2012-05, Vol.51 (19), p.4618-4622
Hauptverfasser:	Schiro, Perry G., Zhao, Mengxia, Kuo, Jason S., Koehler, Karen M., Sabath, Daniel E., Chiu, Daniel T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies - immunology Antigens, Neoplasm - immunology Antigens, Neoplasm - metabolism Blood Cells - cytology cancer Cell Adhesion Molecules - immunology Cell Adhesion Molecules - metabolism cell capture cells Epithelial Cell Adhesion Molecule Flow Cytometry Fluorescent Dyes - chemistry high-throughput screening High-Throughput Screening Assays Humans Microscopy, Confocal Neoplastic Cells, Circulating Neoplastic Stem Cells - cytology Neoplastic Stem Cells - metabolism Receptor, ErbB-2 - immunology Receptor, ErbB-2 - metabolism single-cell analysis
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Zusammenfassung:	Enrichment of cells: An approach called ensemble‐decision aliquot ranking (eDAR) for isolating rare cells from peripheral blood is described. The eDAR process has a recovery of over 93 % (number of runs n=9) with a zero false positive rate (n=8) and provides direct easy access to individual isolated live cells for downstream single‐cell manipulation and analysis (CTCs=circulating tumor cells).
ISSN:	1433-7851 1521-3773 1521-3773
DOI:	10.1002/anie.201108695