Time-gated luminescence microscopy with responsive nonmetal probes for mapping activity of protein kinases in living cells

Time-gated luminescence microscopy with responsive nonmetal probes for mapping activity of protein kinases in living cells

A photoluminescence probe ARC-1185, possessing both high affinity towards basophilic protein kinases (PKs) and microsecond-scale luminescence lifetime when associated with a kinase, was used for the mapping of ARC-1185-PK complexes in living cells with time-gated luminescence microscopy.

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Bibliographische Detailangaben
Veröffentlicht in:Chemical communications (Cambridge, England) England), 2012-09, Vol.48 (68), p.8595-8597
Hauptverfasser: Vaasa, Angela, Ligi, Kadri, Mohandessi, Shabnam, Enkvist, Erki, Uri, Asko, Miller, Lawrence W
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Basophils - enzymology
Dogs
Fluorescent Dyes - chemistry
Madin Darby Canine Kidney Cells
Microscopy, Fluorescence
Oligopeptides - chemistry
Protein Kinases - chemistry
Protein Kinases - metabolism
Time Factors
Ultraviolet Rays
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Beschreibung
Zusammenfassung:A photoluminescence probe ARC-1185, possessing both high affinity towards basophilic protein kinases (PKs) and microsecond-scale luminescence lifetime when associated with a kinase, was used for the mapping of ARC-1185-PK complexes in living cells with time-gated luminescence microscopy.
ISSN:1359-7345
1364-548X
DOI:10.1039/c2cc33565d