In vitro transcription with extracts of nuclei of Drosophila embryos

Due to an inadvertant error, the sentence on page 2726, eighth line from the bottom: ‘After pyrophosphatase treatment, decapped transcripts now adsorbed no longer to the second dihydroxyboronyl-sqtharosc-column…’ should be replaced by: ‘After pyrophosphatase treatment, decapped transcripts should no...

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Veröffentlicht in:Nucleic acids research 1985-05, Vol.13 (10), p.3790-3790
Hauptverfasser: Heiermann, R., Pongs, O.
Format: Artikel
Sprache:eng
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Zusammenfassung:Due to an inadvertant error, the sentence on page 2726, eighth line from the bottom: ‘After pyrophosphatase treatment, decapped transcripts now adsorbed no longer to the second dihydroxyboronyl-sqtharosc-column…’ should be replaced by: ‘After pyrophosphatase treatment, decapped transcripts should not adsorb to the second dihydroxyboronyl-sepharose-column. Now 80% of the material flowed through the column (Fig. 10, lanes 2 and 3), whereas not more than 50% of the material flowed through the column without pymphosphatase treatment.’
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/13.10.3790