Optimizing the expression in E. coli of a synthetic gene encoding somatomedin-C (IGF-I)

Double-stranded DNA encoding the human hormone somatomedin-C (SMC) has been synthesized. This synthetic gene hae been inserted into a plasmid bearing the strong leftward promoter (PL) of bacteriophage λ and expressed in E. coli. Codons for the N-terminal region of SMC which maximized the hormone...

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Veröffentlicht in:	Nucleic acids research 1985-03, Vol.13 (6), p.1923-1938
Hauptverfasser:	Buell, Gary, Schulz, Marie-Francoise, Selzer, Gerald, Chollet, Andre, Movva, N.Rao, Semon, Dominique, Escancz, Sonia, Kawashima, Eric
Format:	Artikel
Sprache:	eng
Schlagworte:	ATP-Dependent Proteases Cloning, Molecular DNA, Recombinant Endopeptidases - genetics Escherichia coli Escherichia coli - genetics Escherichia coli Proteins Gene Expression Regulation Genes Genes, Bacterial Heat-Shock Proteins - genetics Insulin-Like Growth Factor I Mutation Nucleic Acid Conformation Promoter Regions, Genetic Protease La Serine Endopeptidases Somatomedins - genetics
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Beschreibung
Zusammenfassung:	Double-stranded DNA encoding the human hormone somatomedin-C (SMC) has been synthesized. This synthetic gene hae been inserted into a plasmid bearing the strong leftward promoter (PL) of bacteriophage λ and expressed in E. coli. Codons for the N-terminal region of SMC which maximized the hormone's synthesis were chosen in an SMC-lac z fusion assay. The amounts of SMC accumulated in E. coli were influenced by mutations at two chromosomal loci, lon and htpR.
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/13.6.1923