Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family
We have isolated and sequenced a full-length cDNA clone encoding rat glyceraldehyde-3-phosphate-dehydrogenase (GAPDH, E.C.1.2.1.12). The entire mRNA is 1269 nucleotides long exclusive of poly(A) and contains respectively 71 and 196 bases of 5′ and 3′ non-coding regions. Primer extension as well as S...
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| Veröffentlicht in: | Nucleic acids research 1985-03, Vol.13 (5), p.1431-1442 |
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| container_title | Nucleic acids research |
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| creator | Fort, Ph Marty, L. Piechaczyk, M. Sabrouty, S.El Dani, Ch Jeanteur, Ph Blanchard, J.M. |
| description | We have isolated and sequenced a full-length cDNA clone encoding rat glyceraldehyde-3-phosphate-dehydrogenase (GAPDH, E.C.1.2.1.12). The entire mRNA is 1269 nucleotides long exclusive of poly(A) and contains respectively 71 and 196 bases of 5′ and 3′ non-coding regions. Primer extension as well as S1 nuclease protection experiments clearly established that a single (or at least a highly prominent) GAPDH mRNA species is expressed in all rat tissues examined. This sequence allowed the determination of the hitherto unknown primary structure of rat GAPDH which is 333 aninoacids long. Comparison between GAPDH sequences from rat, man and chicken revealed a high degree of sequence conservation at both nucleotide and protein levels. |
| doi_str_mv | 10.1093/nar/13.5.1431 |
| format | Article |
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The entire mRNA is 1269 nucleotides long exclusive of poly(A) and contains respectively 71 and 196 bases of 5′ and 3′ non-coding regions. Primer extension as well as S1 nuclease protection experiments clearly established that a single (or at least a highly prominent) GAPDH mRNA species is expressed in all rat tissues examined. This sequence allowed the determination of the hitherto unknown primary structure of rat GAPDH which is 333 aninoacids long. Comparison between GAPDH sequences from rat, man and chicken revealed a high degree of sequence conservation at both nucleotide and protein levels.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/13.5.1431</identifier><identifier>PMID: 2987824</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biochemistry, Molecular Biology ; Biodiversity ; Cancer ; cDNA ; Cellular Biology ; Chickens ; Dentistry ; Development Biology ; DNA ; DNA - analysis ; Embryology and Organogenesis ; Endonucleases ; Endonucleases - metabolism ; gene expression ; Gene Expression Regulation ; Glyceraldehyde-3-Phosphate Dehydrogenases ; Glyceraldehyde-3-Phosphate Dehydrogenases - genetics ; glyceraldehyde-phosphate dehydrogenase ; Humans ; Life Sciences ; Molecular biology ; mRNA ; Nucleic Acid Conformation ; nucleotide sequence ; Rats ; Rats, Inbred Strains ; RNA, Messenger ; RNA, Messenger - analysis ; Single-Strand Specific DNA and RNA Endonucleases ; Subcellular Processes ; Systematics, Phylogenetics and taxonomy</subject><ispartof>Nucleic acids research, 1985-03, Vol.13 (5), p.1431-1442</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4301-91307163412a2aec6a2d41527d47b853052350a50b2f9871fe569f3dd13b0f013</citedby><orcidid>0000-0001-5997-8722 ; 0000-0003-3228-0230</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC341087/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC341087/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2987824$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-02267584$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Fort, Ph</creatorcontrib><creatorcontrib>Marty, L.</creatorcontrib><creatorcontrib>Piechaczyk, M.</creatorcontrib><creatorcontrib>Sabrouty, S.El</creatorcontrib><creatorcontrib>Dani, Ch</creatorcontrib><creatorcontrib>Jeanteur, Ph</creatorcontrib><creatorcontrib>Blanchard, J.M.</creatorcontrib><title>Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>We have isolated and sequenced a full-length cDNA clone encoding rat glyceraldehyde-3-phosphate-dehydrogenase (GAPDH, E.C.1.2.1.12). The entire mRNA is 1269 nucleotides long exclusive of poly(A) and contains respectively 71 and 196 bases of 5′ and 3′ non-coding regions. Primer extension as well as S1 nuclease protection experiments clearly established that a single (or at least a highly prominent) GAPDH mRNA species is expressed in all rat tissues examined. This sequence allowed the determination of the hitherto unknown primary structure of rat GAPDH which is 333 aninoacids long. Comparison between GAPDH sequences from rat, man and chicken revealed a high degree of sequence conservation at both nucleotide and protein levels.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biochemistry, Molecular Biology</subject><subject>Biodiversity</subject><subject>Cancer</subject><subject>cDNA</subject><subject>Cellular Biology</subject><subject>Chickens</subject><subject>Dentistry</subject><subject>Development Biology</subject><subject>DNA</subject><subject>DNA - analysis</subject><subject>Embryology and Organogenesis</subject><subject>Endonucleases</subject><subject>Endonucleases - metabolism</subject><subject>gene expression</subject><subject>Gene Expression Regulation</subject><subject>Glyceraldehyde-3-Phosphate Dehydrogenases</subject><subject>Glyceraldehyde-3-Phosphate Dehydrogenases - genetics</subject><subject>glyceraldehyde-phosphate dehydrogenase</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Molecular biology</subject><subject>mRNA</subject><subject>Nucleic Acid Conformation</subject><subject>nucleotide sequence</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>RNA, Messenger</subject><subject>RNA, Messenger - analysis</subject><subject>Single-Strand Specific DNA and RNA Endonucleases</subject><subject>Subcellular Processes</subject><subject>Systematics, Phylogenetics and taxonomy</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFksuP0zAQhy0EWsrCkSOST0gc0vX4nQOHagWUVQUCAUJcLDdxGi9JHOx0tT3yn-PSqjwue7GtmW-e_iH0FMgcSMkuBhsvgM3FHDiDe2gGTNKCl5LeRzPCiCiAcP0QPUrpmhDgIPgZOqOlVpryGfr5xUYftglHO2Fbb7sJTz6lrUvY3Y7RpYTD0O3y4XBvr0PE_cd3C5xGV_nMNDH0eGod3nS7ykXb1a7d1a5gxdiGNLZ2csVvUwwbN9iUk-QSPr99hRvb-273GD1obJfck-N9jj6_fvXpclms3r95e7lYFRVnBIoSGFEgGQdqqXWVtLTOw1BVc7XWIg9KmSBWkDVt8nDQOCHLhtU1sDVpCLBz9PKQd9yue1dXbphyv2aMvrdxZ4L15l_P4FuzCTcmlyRa5fgXh_j2v6jlYmX2NkKpVELzm32t58daMfzIu5xM71Plus4OLi_bKAks_1Z5J5gRXhKq7wa5JEKDyGBxAKsYUoquOfUKxOwFY7JgDDAjzF4wmX_291pO9FEhf_L5NLnbk9vG70YqpoRZfv1mPiz11ZWS3Gj2C-mvzFs</recordid><startdate>19850311</startdate><enddate>19850311</enddate><creator>Fort, Ph</creator><creator>Marty, L.</creator><creator>Piechaczyk, M.</creator><creator>Sabrouty, S.El</creator><creator>Dani, Ch</creator><creator>Jeanteur, Ph</creator><creator>Blanchard, J.M.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5997-8722</orcidid><orcidid>https://orcid.org/0000-0003-3228-0230</orcidid></search><sort><creationdate>19850311</creationdate><title>Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family</title><author>Fort, Ph ; Marty, L. ; Piechaczyk, M. ; Sabrouty, S.El ; Dani, Ch ; Jeanteur, Ph ; Blanchard, J.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4301-91307163412a2aec6a2d41527d47b853052350a50b2f9871fe569f3dd13b0f013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biochemistry, Molecular Biology</topic><topic>Biodiversity</topic><topic>Cancer</topic><topic>cDNA</topic><topic>Cellular Biology</topic><topic>Chickens</topic><topic>Dentistry</topic><topic>Development Biology</topic><topic>DNA</topic><topic>DNA - analysis</topic><topic>Embryology and Organogenesis</topic><topic>Endonucleases</topic><topic>Endonucleases - metabolism</topic><topic>gene expression</topic><topic>Gene Expression Regulation</topic><topic>Glyceraldehyde-3-Phosphate Dehydrogenases</topic><topic>Glyceraldehyde-3-Phosphate Dehydrogenases - genetics</topic><topic>glyceraldehyde-phosphate dehydrogenase</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Molecular biology</topic><topic>mRNA</topic><topic>Nucleic Acid Conformation</topic><topic>nucleotide sequence</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>RNA, Messenger</topic><topic>RNA, Messenger - analysis</topic><topic>Single-Strand Specific DNA and RNA Endonucleases</topic><topic>Subcellular Processes</topic><topic>Systematics, Phylogenetics and taxonomy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fort, Ph</creatorcontrib><creatorcontrib>Marty, L.</creatorcontrib><creatorcontrib>Piechaczyk, M.</creatorcontrib><creatorcontrib>Sabrouty, S.El</creatorcontrib><creatorcontrib>Dani, Ch</creatorcontrib><creatorcontrib>Jeanteur, Ph</creatorcontrib><creatorcontrib>Blanchard, J.M.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>Hyper Article en Ligne (HAL) (Open Access)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fort, Ph</au><au>Marty, L.</au><au>Piechaczyk, M.</au><au>Sabrouty, S.El</au><au>Dani, Ch</au><au>Jeanteur, Ph</au><au>Blanchard, J.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1985-03-11</date><risdate>1985</risdate><volume>13</volume><issue>5</issue><spage>1431</spage><epage>1442</epage><pages>1431-1442</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><abstract>We have isolated and sequenced a full-length cDNA clone encoding rat glyceraldehyde-3-phosphate-dehydrogenase (GAPDH, E.C.1.2.1.12). The entire mRNA is 1269 nucleotides long exclusive of poly(A) and contains respectively 71 and 196 bases of 5′ and 3′ non-coding regions. Primer extension as well as S1 nuclease protection experiments clearly established that a single (or at least a highly prominent) GAPDH mRNA species is expressed in all rat tissues examined. This sequence allowed the determination of the hitherto unknown primary structure of rat GAPDH which is 333 aninoacids long. Comparison between GAPDH sequences from rat, man and chicken revealed a high degree of sequence conservation at both nucleotide and protein levels.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>2987824</pmid><doi>10.1093/nar/13.5.1431</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-5997-8722</orcidid><orcidid>https://orcid.org/0000-0003-3228-0230</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Nucleic acids research, 1985-03, Vol.13 (5), p.1431-1442 |
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| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy; PubMed Central |
| subjects | Amino Acid Sequence Animals Base Sequence Biochemistry, Molecular Biology Biodiversity Cancer cDNA Cellular Biology Chickens Dentistry Development Biology DNA DNA - analysis Embryology and Organogenesis Endonucleases Endonucleases - metabolism gene expression Gene Expression Regulation Glyceraldehyde-3-Phosphate Dehydrogenases Glyceraldehyde-3-Phosphate Dehydrogenases - genetics glyceraldehyde-phosphate dehydrogenase Humans Life Sciences Molecular biology mRNA Nucleic Acid Conformation nucleotide sequence Rats Rats, Inbred Strains RNA, Messenger RNA, Messenger - analysis Single-Strand Specific DNA and RNA Endonucleases Subcellular Processes Systematics, Phylogenetics and taxonomy |
| title | Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family |
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