Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family

We have isolated and sequenced a full-length cDNA clone encoding rat glyceraldehyde-3-phosphate-dehydrogenase (GAPDH, E.C.1.2.1.12). The entire mRNA is 1269 nucleotides long exclusive of poly(A) and contains respectively 71 and 196 bases of 5′ and 3′ non-coding regions. Primer extension as well as S...

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Veröffentlicht in:	Nucleic acids research 1985-03, Vol.13 (5), p.1431-1442
Hauptverfasser:	Fort, Ph, Marty, L., Piechaczyk, M., Sabrouty, S.El, Dani, Ch, Jeanteur, Ph, Blanchard, J.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Base Sequence Biochemistry, Molecular Biology Biodiversity Cancer cDNA Cellular Biology Chickens Dentistry Development Biology DNA DNA - analysis Embryology and Organogenesis Endonucleases Endonucleases - metabolism gene expression Gene Expression Regulation Glyceraldehyde-3-Phosphate Dehydrogenases Glyceraldehyde-3-Phosphate Dehydrogenases - genetics glyceraldehyde-phosphate dehydrogenase Humans Life Sciences Molecular biology mRNA Nucleic Acid Conformation nucleotide sequence Rats Rats, Inbred Strains RNA, Messenger RNA, Messenger - analysis Single-Strand Specific DNA and RNA Endonucleases Subcellular Processes Systematics, Phylogenetics and taxonomy
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Zusammenfassung:	We have isolated and sequenced a full-length cDNA clone encoding rat glyceraldehyde-3-phosphate-dehydrogenase (GAPDH, E.C.1.2.1.12). The entire mRNA is 1269 nucleotides long exclusive of poly(A) and contains respectively 71 and 196 bases of 5′ and 3′ non-coding regions. Primer extension as well as S1 nuclease protection experiments clearly established that a single (or at least a highly prominent) GAPDH mRNA species is expressed in all rat tissues examined. This sequence allowed the determination of the hitherto unknown primary structure of rat GAPDH which is 333 aninoacids long. Comparison between GAPDH sequences from rat, man and chicken revealed a high degree of sequence conservation at both nucleotide and protein levels.
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/13.5.1431