Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation
How to click with biomolecules: Copper‐catalyzed azide–alkyne cycloaddition has been optimized for use with biological molecules. The key development is the addition of two reagents that allow ascorbate to be used as reducing agent whilst eliminating problems caused by copper ascorbate side reaction...
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| Veröffentlicht in: | Angewandte Chemie (International ed.) 2009-01, Vol.48 (52), p.9879-9883 |
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| container_issue | 52 |
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| container_title | Angewandte Chemie (International ed.) |
| container_volume | 48 |
| creator | Hong, Vu Presolski, Stanislav I Ma, Celia Finn, M.G |
| description | How to click with biomolecules: Copper‐catalyzed azide–alkyne cycloaddition has been optimized for use with biological molecules. The key development is the addition of two reagents that allow ascorbate to be used as reducing agent whilst eliminating problems caused by copper ascorbate side reactions. The result is a robust, rapid, and convenient procedure for the modification of proteins, DNA, RNA, and other biomolecules (see scheme). |
| doi_str_mv | 10.1002/anie.200905087 |
| format | Article |
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The key development is the addition of two reagents that allow ascorbate to be used as reducing agent whilst eliminating problems caused by copper ascorbate side reactions. The result is a robust, rapid, and convenient procedure for the modification of proteins, DNA, RNA, and other biomolecules (see scheme).</description><identifier>ISSN: 1433-7851</identifier><identifier>EISSN: 1521-3773</identifier><identifier>DOI: 10.1002/anie.200905087</identifier><identifier>PMID: 19943299</identifier><language>eng</language><publisher>Weinheim: Wiley-VCH Verlag</publisher><subject>alkynes ; Alkynes - chemistry ; Allolevivirus - chemistry ; Ascorbic Acid - chemistry ; azides ; Azides - chemistry ; bioconjugation ; Catalysis ; copper ; Copper - chemistry ; cycloaddition ; RNA - chemistry ; Serum Albumin, Bovine - chemistry</subject><ispartof>Angewandte Chemie (International ed.), 2009-01, Vol.48 (52), p.9879-9883</ispartof><rights>Copyright © 2009 WILEY‐VCH Verlag GmbH & Co. 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| fulltext | fulltext |
| identifier | ISSN: 1433-7851 |
| ispartof | Angewandte Chemie (International ed.), 2009-01, Vol.48 (52), p.9879-9883 |
| issn | 1433-7851 1521-3773 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3410708 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | alkynes Alkynes - chemistry Allolevivirus - chemistry Ascorbic Acid - chemistry azides Azides - chemistry bioconjugation Catalysis copper Copper - chemistry cycloaddition RNA - chemistry Serum Albumin, Bovine - chemistry |
| title | Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation |
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