Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation

Analysis and Optimization of Copper-Catalyzed Azide-Alkyne Cycloaddition for Bioconjugation

How to click with biomolecules: Copper‐catalyzed azide–alkyne cycloaddition has been optimized for use with biological molecules. The key development is the addition of two reagents that allow ascorbate to be used as reducing agent whilst eliminating problems caused by copper ascorbate side reaction...

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Veröffentlicht in:Angewandte Chemie (International ed.) 2009-01, Vol.48 (52), p.9879-9883
Hauptverfasser: Hong, Vu, Presolski, Stanislav I, Ma, Celia, Finn, M.G
Format: Artikel
Sprache:eng
Schlagworte:
alkynes
Alkynes - chemistry
Allolevivirus - chemistry
Ascorbic Acid - chemistry
azides
Azides - chemistry
bioconjugation
Catalysis
copper
Copper - chemistry
cycloaddition
RNA - chemistry
Serum Albumin, Bovine - chemistry
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Zusammenfassung:How to click with biomolecules: Copper‐catalyzed azide–alkyne cycloaddition has been optimized for use with biological molecules. The key development is the addition of two reagents that allow ascorbate to be used as reducing agent whilst eliminating problems caused by copper ascorbate side reactions. The result is a robust, rapid, and convenient procedure for the modification of proteins, DNA, RNA, and other biomolecules (see scheme).
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200905087