Primary structure of the soybean nodulin-23 gene and potential regulatory elements in the 5'-flanking regions of nodulin and leghemoglobin genes

The nodulin-23 gene of soybean is one of the most abundantly transcribed genes induced during symbiosis with Rhizobium. Using a plasmid (pNod25) from a nodule cDNA library, we have isolated the nodulin gene from a soybean genomic library. Nucleotide sequence analysis of the cDNA and of the genomic c...

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Veröffentlicht in:Nucleic acids research 1985-01, Vol.13 (1), p.239-249
Hauptverfasser: Mauro, V.P, Nguyen, T, Katinakis, P, Verma, D.P.S
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Sprache:eng
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Zusammenfassung:The nodulin-23 gene of soybean is one of the most abundantly transcribed genes induced during symbiosis with Rhizobium. Using a plasmid (pNod25) from a nodule cDNA library, we have isolated the nodulin gene from a soybean genomic library. Nucleotide sequence analysis of the cDNA and of the genomic clone indicated that the coding region of this gene is 669 bp long and is interrupted by a single intron of about 53ø bp. The deduced protein sequence suggests that nodulin-23 may have a signal sequence. The 5′-flanking sequence of two other nodulin genes, nodulin-24 encoding for a mambrane polypeptide and one of the leghanoglobin genes (LbC3), were obtained. Comparison of these sequences revealed three conserved eg ions, one of which, an octanucleotide (GTTTCCCT), has 1øø% homology. The conserved sequences are arranged in a unique fashion and have a spatial organization with respect to order and position, which nay suggest a potential regulatory role in controlling the expression of nodulin and leghanoglobin genes during symbiosis.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/13.1.239