Isolation and sequence analysis of a full length cDNA clone coding for human mitochondrial aldehyde dehydrogenase

A partial cDNA clone coding for the mitochondrial isozyme (ALDH I) was isolated from a human liver cDNA library (1). Using an ALDH I cDNA fragment, the authors have isolated a full length cDNA clone coding for ALDH I from a human fetal muscle cDNA library. The lambda cAL123 clone comprised the whole...

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Veröffentlicht in:	Nucleic acids research 1987-04, Vol.15 (7), p.3179-3179
Hauptverfasser:	BRAUN, T, BOBER, E, SURJIT SINGH, AGARWAL, D. P, GOEDDE, H. W
Format:	Artikel
Sprache:	eng
Schlagworte:	aldehyde dehydrogenase Aldehyde Dehydrogenase - genetics Amino Acid Sequence Base Sequence Biological and medical sciences Biotechnology cDNA Cloning, Molecular DNA - metabolism Fundamental and applied biological sciences. Psychology Genes Genetic engineering Genetic technics Humans man Methods. Procedures. Technologies mitochondria Mitochondria, Liver - enzymology Molecular cloning muscles nucleotide sequence
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Zusammenfassung:	A partial cDNA clone coding for the mitochondrial isozyme (ALDH I) was isolated from a human liver cDNA library (1). Using an ALDH I cDNA fragment, the authors have isolated a full length cDNA clone coding for ALDH I from a human fetal muscle cDNA library. The lambda cAL123 clone comprised the whole coding region of ALDH I mRNA and the adjacent non-coding regions as established by sequence analysis. The deduced amino acid sequence of ALDH I showed a homology of approximately 70% to the known amino acid sequence of the cytoplasmic isozyme (ALDH II).
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/15.7.3179