An evaluation of urinary microRNA reveals a high sensitivity for bladder cancer

Background: Urinary biomarkers are needed to improve the care and reduce the cost of managing bladder cancer. Current biomarkers struggle to identify both high and low-grade cancers due to differing molecular pathways. Changes in microRNA (miR) expression are seen in urothelial carcinogenesis in a p...

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Veröffentlicht in:	British journal of cancer 2012-06, Vol.107 (1), p.123-128
Hauptverfasser:	Miah, S, Dudziec, E, Drayton, R M, Zlotta, A R, Morgan, S L, Rosario, D J, Hamdy, F C, Catto, J W F
Format:	Artikel
Sprache:	eng
Schlagworte:	631/337/384/331 692/699/67/589/1336 Adolescent Adult Aged Aged, 80 and over Biological and medical sciences Biomarkers, Tumor - urine Biomedical and Life Sciences Biomedicine Bladder cancer Cancer Research Drug Resistance Epidemiology Female Humans Male Medical sciences MicroRNAs - urine Middle Aged Molecular Diagnostics Molecular Medicine Nephrology. Urinary tract diseases Oncology Sensitivity and Specificity Tumors Tumors of the urinary system Urinary Bladder Neoplasms - diagnosis Urinary Bladder Neoplasms - urine Urinary tract. Prostate gland Young Adult
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Zusammenfassung:	Background: Urinary biomarkers are needed to improve the care and reduce the cost of managing bladder cancer. Current biomarkers struggle to identify both high and low-grade cancers due to differing molecular pathways. Changes in microRNA (miR) expression are seen in urothelial carcinogenesis in a phenotype-specific manner. We hypothesised that urinary miRs reflecting low- and high-grade pathways could detect bladder cancers and overcome differences in genetic events seen within the disease. Methods: We investigated urinary samples ( n =121) from patients with bladder cancer ( n =68) and age-matched controls ( n =53). Fifteen miRs were quantified using real-time PCR. Results: We found that miR is stable within urinary cells despite adverse handling and detected differential expression of 10 miRs from patients with cancer and controls (miRs−15a/15b/24-1/27b/100/135b/203/212/328/1224, ANOVA P
ISSN:	0007-0920 1532-1827
DOI:	10.1038/bjc.2012.221