Analysis of Bacterial Community in the Ginseng Soil Using Denaturing Gradient Gel Electrophoresis (DGGE)

Analysis of Bacterial Community in the Ginseng Soil Using Denaturing Gradient Gel Electrophoresis (DGGE)

The objective of this work was to determine the shifts in the PCR-DGGE profiles of bacterial communities associated with the rhizosphere soil of ginseng at varying age levels. Differences in the dominance of intense DNA bands in the DGGE profile was observed over the age of the plants indicating the...

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Veröffentlicht in:Indian journal of microbiology 2012-06, Vol.52 (2), p.286-288
Hauptverfasser: Vendan, Regupathy Thamizh, Lee, Sun Hee, Yu, Young Joon, Rhee, Young Ha
Format: Artikel
Sprache:eng
Schlagworte:
Actinobacteria
Age
bacterial communities
Biomedical and Life Sciences
Cloning
Community structure
denaturing gradient gel electrophoresis
DNA
Life Sciences
Medical Microbiology
Microbiology
Panax
plant age
Rhizobiales
Rhizosphere
Short Communication
soil
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Zusammenfassung:The objective of this work was to determine the shifts in the PCR-DGGE profiles of bacterial communities associated with the rhizosphere soil of ginseng at varying age levels. Differences in the dominance of intense DNA bands in the DGGE profile was observed over the age of the plants indicating the fluctuation in the microbial community structure. The bacterial orders of actinomycetales of Actinobacteria and Spingomonadales and Rhizobiales of α-Proteobacteria were predominant in the ginseng soil.
ISSN:0046-8991
0973-7715
DOI:10.1007/s12088-011-0193-3