Nucleotide sequence of cDNA encoding the catalytic subunit of phosphorylase kinase from rat soleus muscle

The authors have cloned a cDNA which contains the complete coding region for the gamma subunit of phosphorylase kinase from a lambda gt10 library prepared from adult rat soleus muscle. The library was screened with a 75 bp oligonucleotide probe, the sequence of which was based on the amino acid sequence of the rabbit gamma subunit employing empirically derived codon usage rules. The probe was directed against a region believed to be unique to phosphorylase kinase and not shared by other kinases. The probe proved to be 87% homologous with the corresponding nucleotide sequence in the cDNA.