Initial crystallographic studies of a small heat-shock protein from Xylella fastidiosa

The ORF XF2234 in the Xylella fastidiosa genome was identified as encoding a small heat‐shock protein of 17.9 kDa (HSP17.9). HSP17.9 was found as one of the proteins that are induced during X. fastidiosa proliferation and infection in citrus culture. Recombinant HSP17.9 was crystallized and surface...

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Veröffentlicht in:Acta crystallographica. Section F, Structural biology and crystallization communications Structural biology and crystallization communications, 2012-05, Vol.68 (5), p.535-539
Hauptverfasser: Tada, Susely F. S., Saraiva, Antonio Marcos, Lorite, Gabriela S., Rosselli-Murai, Luciana K., Pelloso, Alexandre César, dos Santos, Marcelo Leite, Trivella, Daniela B. B., Cotta, Mônica A., de Souza, Anete Pereira, Aparicio, Ricardo
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Sprache:eng
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Zusammenfassung:The ORF XF2234 in the Xylella fastidiosa genome was identified as encoding a small heat‐shock protein of 17.9 kDa (HSP17.9). HSP17.9 was found as one of the proteins that are induced during X. fastidiosa proliferation and infection in citrus culture. Recombinant HSP17.9 was crystallized and surface atomic force microscopy experiments were conducted with the aim of better characterizing the HSP17.9 crystals. X‐ray diffraction data were collected at 2.7 Å resolution. The crystal belonged to space group P4322, with unit‐cell parameters a = 68.90, b = 68.90, c = 72.51 Å, and is the first small heat‐shock protein to crystallize in this space group.
ISSN:1744-3091
1744-3091
2053-230X
DOI:10.1107/S1744309112009347