Rapid DNA fingerprinting using alkaline phosphatase-conjugated oligonucleotides

DNA fingerprinting is a powerful technique with applications in genetic mapping, population biology, and forensic medicine. By detecting multiple, highly-variable mini-satellite DNA polymorphisms, it is capable of identifying individuals with virtual certainty. Radio-labeled probes, however, limit the use of the technique to research and specialized clinical laboratories. The authors describe here the use of an alkaline phosphatase-conjugated oligonucleotide probe and colorimetric detection for determining DNA fingerprints.