Light-Activated Gene Editing with a Photocaged Zinc-Finger Nuclease
Light, zinc‐finger nuclease, action: Using unnatural amino acid mutagenesis, a light‐activated zinc‐finger nuclease enzyme was engineered through incorporation of a photocaged tyrosine residue at a DNA–protein interface. The caged zinc‐finger nuclease was completely inactive until irradiated with UV...
Gespeichert in:
| Veröffentlicht in: | Angewandte Chemie International Edition 2011-07, Vol.50 (30), p.6839-6842 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 6842 |
|---|---|
| container_issue | 30 |
| container_start_page | 6839 |
| container_title | Angewandte Chemie International Edition |
| container_volume | 50 |
| creator | Chou, Chungjung Deiters, Alexander |
| description | Light, zinc‐finger nuclease, action: Using unnatural amino acid mutagenesis, a light‐activated zinc‐finger nuclease enzyme was engineered through incorporation of a photocaged tyrosine residue at a DNA–protein interface. The caged zinc‐finger nuclease was completely inactive until irradiated with UV light, thus enabling photochemical control of sequence specific gene editing in mammalian cells (see picture). |
| doi_str_mv | 10.1002/anie.201101157 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3367882</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3276987711</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5057-771fc417d069294e444dd1a6ff0f532ece5cca5ba8e69114e95f58bd39f54a183</originalsourceid><addsrcrecordid>eNqFkM1rGzEQxUVpaVIn1x7LQs_raPS9l4IxthMwbg5tA7kIWTtrK3V2E62cj_--Mk5McioMaOD93tPwCPkKdAiUsjPXBhwyCpBH6g_kGCSDkmvNP-ZdcF5qI-GIfOn7m8wbQ9VncsRAaeCgjsl4HlbrVI58Cg8uYV3MsMViUocU2lXxGNK6cMXlukudd6ssX4fWl9OsYSwWW79B1-MJ-dS4TY-nL--A_J5Ofo3Py_nP2cV4NC-9pFKXWkPjBeiaqopVAoUQdQ1ONQ1tJGfoUXrv5NIZVBWAwEo20ixrXjVSODB8QH7sc--2y1usPbYpuo29i-HWxWfbuWDfK21Y21X3YDlX2hiWA76_BMTufot9sjfdNrb5ZgsSlGImN5Sp4Z7ysev7iM3hB6B2V7rdlW4PpWfDt7d3HfDXljNQ7YHHsMHn_8TZ0eJi8ja83HtDn_Dp4HXxr1Waa2mvFjMr4M-czcbn9pL_A0oDnP4</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1516628028</pqid></control><display><type>article</type><title>Light-Activated Gene Editing with a Photocaged Zinc-Finger Nuclease</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Chou, Chungjung ; Deiters, Alexander</creator><creatorcontrib>Chou, Chungjung ; Deiters, Alexander</creatorcontrib><description>Light, zinc‐finger nuclease, action: Using unnatural amino acid mutagenesis, a light‐activated zinc‐finger nuclease enzyme was engineered through incorporation of a photocaged tyrosine residue at a DNA–protein interface. The caged zinc‐finger nuclease was completely inactive until irradiated with UV light, thus enabling photochemical control of sequence specific gene editing in mammalian cells (see picture).</description><edition>International ed. in English</edition><identifier>ISSN: 1433-7851</identifier><identifier>EISSN: 1521-3773</identifier><identifier>DOI: 10.1002/anie.201101157</identifier><identifier>PMID: 21671316</identifier><identifier>CODEN: ACIEAY</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>caged compounds ; Endonucleases - genetics ; Endonucleases - metabolism ; Escherichia coli - genetics ; gene technology ; HEK293 Cells ; Humans ; light-activation ; Models, Molecular ; Mutagenesis ; Photochemical Processes ; Protein Engineering ; Tyrosine - genetics ; Ultraviolet Rays ; Zinc Fingers ; zinc-finger nucleases</subject><ispartof>Angewandte Chemie International Edition, 2011-07, Vol.50 (30), p.6839-6842</ispartof><rights>Copyright © 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2011 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5057-771fc417d069294e444dd1a6ff0f532ece5cca5ba8e69114e95f58bd39f54a183</citedby><cites>FETCH-LOGICAL-c5057-771fc417d069294e444dd1a6ff0f532ece5cca5ba8e69114e95f58bd39f54a183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fanie.201101157$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fanie.201101157$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21671316$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chou, Chungjung</creatorcontrib><creatorcontrib>Deiters, Alexander</creatorcontrib><title>Light-Activated Gene Editing with a Photocaged Zinc-Finger Nuclease</title><title>Angewandte Chemie International Edition</title><addtitle>Angew. Chem. Int. Ed</addtitle><description>Light, zinc‐finger nuclease, action: Using unnatural amino acid mutagenesis, a light‐activated zinc‐finger nuclease enzyme was engineered through incorporation of a photocaged tyrosine residue at a DNA–protein interface. The caged zinc‐finger nuclease was completely inactive until irradiated with UV light, thus enabling photochemical control of sequence specific gene editing in mammalian cells (see picture).</description><subject>caged compounds</subject><subject>Endonucleases - genetics</subject><subject>Endonucleases - metabolism</subject><subject>Escherichia coli - genetics</subject><subject>gene technology</subject><subject>HEK293 Cells</subject><subject>Humans</subject><subject>light-activation</subject><subject>Models, Molecular</subject><subject>Mutagenesis</subject><subject>Photochemical Processes</subject><subject>Protein Engineering</subject><subject>Tyrosine - genetics</subject><subject>Ultraviolet Rays</subject><subject>Zinc Fingers</subject><subject>zinc-finger nucleases</subject><issn>1433-7851</issn><issn>1521-3773</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1rGzEQxUVpaVIn1x7LQs_raPS9l4IxthMwbg5tA7kIWTtrK3V2E62cj_--Mk5McioMaOD93tPwCPkKdAiUsjPXBhwyCpBH6g_kGCSDkmvNP-ZdcF5qI-GIfOn7m8wbQ9VncsRAaeCgjsl4HlbrVI58Cg8uYV3MsMViUocU2lXxGNK6cMXlukudd6ssX4fWl9OsYSwWW79B1-MJ-dS4TY-nL--A_J5Ofo3Py_nP2cV4NC-9pFKXWkPjBeiaqopVAoUQdQ1ONQ1tJGfoUXrv5NIZVBWAwEo20ixrXjVSODB8QH7sc--2y1usPbYpuo29i-HWxWfbuWDfK21Y21X3YDlX2hiWA76_BMTufot9sjfdNrb5ZgsSlGImN5Sp4Z7ysev7iM3hB6B2V7rdlW4PpWfDt7d3HfDXljNQ7YHHsMHn_8TZ0eJi8ja83HtDn_Dp4HXxr1Waa2mvFjMr4M-czcbn9pL_A0oDnP4</recordid><startdate>20110718</startdate><enddate>20110718</enddate><creator>Chou, Chungjung</creator><creator>Deiters, Alexander</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>K9.</scope><scope>5PM</scope></search><sort><creationdate>20110718</creationdate><title>Light-Activated Gene Editing with a Photocaged Zinc-Finger Nuclease</title><author>Chou, Chungjung ; Deiters, Alexander</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5057-771fc417d069294e444dd1a6ff0f532ece5cca5ba8e69114e95f58bd39f54a183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>caged compounds</topic><topic>Endonucleases - genetics</topic><topic>Endonucleases - metabolism</topic><topic>Escherichia coli - genetics</topic><topic>gene technology</topic><topic>HEK293 Cells</topic><topic>Humans</topic><topic>light-activation</topic><topic>Models, Molecular</topic><topic>Mutagenesis</topic><topic>Photochemical Processes</topic><topic>Protein Engineering</topic><topic>Tyrosine - genetics</topic><topic>Ultraviolet Rays</topic><topic>Zinc Fingers</topic><topic>zinc-finger nucleases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chou, Chungjung</creatorcontrib><creatorcontrib>Deiters, Alexander</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Angewandte Chemie International Edition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chou, Chungjung</au><au>Deiters, Alexander</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Light-Activated Gene Editing with a Photocaged Zinc-Finger Nuclease</atitle><jtitle>Angewandte Chemie International Edition</jtitle><addtitle>Angew. Chem. Int. Ed</addtitle><date>2011-07-18</date><risdate>2011</risdate><volume>50</volume><issue>30</issue><spage>6839</spage><epage>6842</epage><pages>6839-6842</pages><issn>1433-7851</issn><eissn>1521-3773</eissn><coden>ACIEAY</coden><abstract>Light, zinc‐finger nuclease, action: Using unnatural amino acid mutagenesis, a light‐activated zinc‐finger nuclease enzyme was engineered through incorporation of a photocaged tyrosine residue at a DNA–protein interface. The caged zinc‐finger nuclease was completely inactive until irradiated with UV light, thus enabling photochemical control of sequence specific gene editing in mammalian cells (see picture).</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>21671316</pmid><doi>10.1002/anie.201101157</doi><tpages>4</tpages><edition>International ed. in English</edition><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1433-7851 |
| ispartof | Angewandte Chemie International Edition, 2011-07, Vol.50 (30), p.6839-6842 |
| issn | 1433-7851 1521-3773 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3367882 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | caged compounds Endonucleases - genetics Endonucleases - metabolism Escherichia coli - genetics gene technology HEK293 Cells Humans light-activation Models, Molecular Mutagenesis Photochemical Processes Protein Engineering Tyrosine - genetics Ultraviolet Rays Zinc Fingers zinc-finger nucleases |
| title | Light-Activated Gene Editing with a Photocaged Zinc-Finger Nuclease |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T21%3A35%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Light-Activated%20Gene%20Editing%20with%20a%20Photocaged%20Zinc-Finger%20Nuclease&rft.jtitle=Angewandte%20Chemie%20International%20Edition&rft.au=Chou,%20Chungjung&rft.date=2011-07-18&rft.volume=50&rft.issue=30&rft.spage=6839&rft.epage=6842&rft.pages=6839-6842&rft.issn=1433-7851&rft.eissn=1521-3773&rft.coden=ACIEAY&rft_id=info:doi/10.1002/anie.201101157&rft_dat=%3Cproquest_pubme%3E3276987711%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1516628028&rft_id=info:pmid/21671316&rfr_iscdi=true |