Light-Activated Gene Editing with a Photocaged Zinc-Finger Nuclease

Light-Activated Gene Editing with a Photocaged Zinc-Finger Nuclease

Light, zinc‐finger nuclease, action: Using unnatural amino acid mutagenesis, a light‐activated zinc‐finger nuclease enzyme was engineered through incorporation of a photocaged tyrosine residue at a DNA–protein interface. The caged zinc‐finger nuclease was completely inactive until irradiated with UV...

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Veröffentlicht in:Angewandte Chemie International Edition 2011-07, Vol.50 (30), p.6839-6842
Hauptverfasser: Chou, Chungjung, Deiters, Alexander
Format: Artikel
Sprache:eng
Schlagworte:
caged compounds
Endonucleases - genetics
Endonucleases - metabolism
Escherichia coli - genetics
gene technology
HEK293 Cells
Humans
light-activation
Models, Molecular
Mutagenesis
Photochemical Processes
Protein Engineering
Tyrosine - genetics
Ultraviolet Rays
Zinc Fingers
zinc-finger nucleases
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Beschreibung
Zusammenfassung:Light, zinc‐finger nuclease, action: Using unnatural amino acid mutagenesis, a light‐activated zinc‐finger nuclease enzyme was engineered through incorporation of a photocaged tyrosine residue at a DNA–protein interface. The caged zinc‐finger nuclease was completely inactive until irradiated with UV light, thus enabling photochemical control of sequence specific gene editing in mammalian cells (see picture).
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201101157