Slit2 Regulates the Dispersal of Oligodendrocyte Precursor Cells via Fyn/RhoA Signaling

Oligodendrocyte precursor cells (OPCs) are a unique type of glia that are responsible for the myelination of the central nervous system. OPC migration is important for myelin formation during central nervous system development and repair. However, the precise extracellular and intracellular mechanisms that regulate OPC migration remain elusive. Slits were reported to regulate neurodevelopmental processes such as migration, adhesion, axon guidance, and elongation through binding to roundabout receptors (Robos). However, the potential roles of Slits/Robos in oligodendrocytes remain unknown. In this study, Slit2 was found to be involved in regulating the dispersal of OPCs through the association between Robo1 and Fyn. Initially, we examined the expression of Robos in OPCs both in vitro and in vivo. Subsequently, the Boyden chamber assay showed that Slit2 could inhibit OPC migration. RoboN, a specific inhibitor of Robos, could significantly attenuate this effect. The effects were confirmed through the explant migration assay. Furthermore, treating OPCs with Slit2 protein deactivated Fyn and increased the level of activated RhoA-GTP. Finally, Fyn was found to form complexes with Robo1, but this association was decreased after Slit2 stimulation. Thus, we demonstrate for the first time that Slit2 regulates the dispersal of oligodendrocyte precursor cells through Fyn and RhoA signaling. Secreted by nervous system midline cells Slits regulate neurodevelopmental processes through binding to roundabout receptors (Robos). Slit2 causes dispersal of oligodendrocyte precursor cells (OPCs) by inducing the association between Robo1 and Fyn. Robo1 interacts with Fyn to repel the migration of OPCs through RhoA activation. Learning how Slit/Robo signaling regulates OPC dispersal is crucial for understanding the distribution of OPCs during central nervous system development.