Gravity-oriented microfluidic device for uniform and massive cell spheroid formation

We propose a simple method for forming massive and uniform three-dimensional (3-D) cell spheroids in a multi-level structured microfluidic device by gravitational force. The concept of orienting the device vertically has allowed spheroid formation, long-term perfusion, and retrieval of the cultured...

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Veröffentlicht in:Biomicrofluidics 2012-03, Vol.6 (1), p.014114-014114-7
Hauptverfasser: Lee, Kangsun, Kim, Choong, Young Yang, Jae, Lee, Hun, Ahn, Byungwook, Xu, Linfeng, Yoon Kang, Ji, Oh, Kwang W.
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Sprache:eng
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Zusammenfassung:We propose a simple method for forming massive and uniform three-dimensional (3-D) cell spheroids in a multi-level structured microfluidic device by gravitational force. The concept of orienting the device vertically has allowed spheroid formation, long-term perfusion, and retrieval of the cultured spheroids by user-friendly standard pipetting. We have successfully formed, perfused, and retrieved uniform, size-controllable, well-conditioned spheroids of human embryonic kidney 293 cells (HEK 293) in the gravity-oriented microfluidic device. We expect the proposed method will be a useful tool to study in-vitro 3-D cell models for the proliferation, differentiation, and metabolism of embryoid bodies or tumours.
ISSN:1932-1058
1932-1058
DOI:10.1063/1.3687409