Efficient DNA isolation within a single gel barrier tube
We previously described, the method in which organic solvents can be separated from aqueous containing DNA by using silica gel tubes (SST tubes). The role of this tube in DNA isolation can be extended further to include performing the SDS-Proteinase K digestion and alcohol precipitation steps, in ad...
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| Veröffentlicht in: | Nucleic acids research 1989-10, Vol.17 (20), p.8393-8393 |
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| container_issue | 20 |
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| container_title | Nucleic acids research |
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| creator | MORENO, R. F BOOTH, F. R HUNG, I. H TILZER, L. L |
| description | We previously described, the method in which organic solvents can be separated from aqueous containing DNA by using silica gel tubes (SST tubes). The role of this tube in DNA isolation can be extended further to include performing the SDS-Proteinase K digestion and alcohol precipitation steps, in addition to the phenol:chloroform extraction. The system is ideal for isolating DNA from large numbers of samples quickly, safely, and easily. |
| doi_str_mv | 10.1093/nar/17.20.8393 |
| format | Article |
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L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficient DNA isolation within a single gel barrier tube</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1989-10-25</date><risdate>1989</risdate><volume>17</volume><issue>20</issue><spage>8393</spage><epage>8393</epage><pages>8393-8393</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>We previously described, the method in which organic solvents can be separated from aqueous containing DNA by using silica gel tubes (SST tubes). The role of this tube in DNA isolation can be extended further to include performing the SDS-Proteinase K digestion and alcohol precipitation steps, in addition to the phenol:chloroform extraction. 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| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1989-10, Vol.17 (20), p.8393-8393 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_334998 |
| source | MEDLINE; PubMed Central; Oxford University Press Journals Digital Archive Legacy |
| subjects | Analytical, structural and metabolic biochemistry Biological and medical sciences Cells, Cultured Centrifugation - methods DNA - blood DNA - isolation & purification DNA Restriction Enzymes Dna, deoxyribonucleoproteins Fundamental and applied biological sciences. Psychology Gels Humans Indicators and Reagents Nucleic acids role |
| title | Efficient DNA isolation within a single gel barrier tube |
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