Efficient DNA isolation within a single gel barrier tube

We previously described, the method in which organic solvents can be separated from aqueous containing DNA by using silica gel tubes (SST tubes). The role of this tube in DNA isolation can be extended further to include performing the SDS-Proteinase K digestion and alcohol precipitation steps, in ad...

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Veröffentlicht in:	Nucleic acids research 1989-10, Vol.17 (20), p.8393-8393
Hauptverfasser:	MORENO, R. F, BOOTH, F. R, HUNG, I. H, TILZER, L. L
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Cells, Cultured Centrifugation - methods DNA - blood DNA - isolation & purification DNA Restriction Enzymes Dna, deoxyribonucleoproteins Fundamental and applied biological sciences. Psychology Gels Humans Indicators and Reagents Nucleic acids role
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Beschreibung
Zusammenfassung:	We previously described, the method in which organic solvents can be separated from aqueous containing DNA by using silica gel tubes (SST tubes). The role of this tube in DNA isolation can be extended further to include performing the SDS-Proteinase K digestion and alcohol precipitation steps, in addition to the phenol:chloroform extraction. The system is ideal for isolating DNA from large numbers of samples quickly, safely, and easily.
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/17.20.8393