Chemical synthesis of the 24 RNA fragments corresponding to hop stunt viroid

A general and practical synthetic method of oligoribonucleotides (10-20 mers) by using the cyanoethyl phosphoramidite approach was described. In this experiment 9-phenylxanthen-9-yl (Pix) and 9-(4-methoxy)phenylxanthen-9-yl (Mox) groups were employed for the 5'-hydroxyls and tetrahydropyranyl (...

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Veröffentlicht in:	Nucleic acids research 1989-10, Vol.17 (20), p.8135-8147
Hauptverfasser:	TANIMURA, H, MAEDA, M, FUKAZAWA, T, SEKINE, M, HATA, T
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Base Sequence Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Indicators and Reagents Magnetic Resonance Spectroscopy Methods. Procedures. Technologies Molecular Sequence Data Nucleic Acid Conformation Nucleic acids Oligoribonucleotides - chemical synthesis Plant Viruses - genetics Rna, ribonucleoproteins RNA, Viral - chemical synthesis Synthetic digonucleotides and genes. Sequencing Viroids - genetics
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Beschreibung
Zusammenfassung:	A general and practical synthetic method of oligoribonucleotides (10-20 mers) by using the cyanoethyl phosphoramidite approach was described. In this experiment 9-phenylxanthen-9-yl (Pix) and 9-(4-methoxy)phenylxanthen-9-yl (Mox) groups were employed for the 5'-hydroxyls and tetrahydropyranyl (Thp) group was used for the 2'-hydroxyl protecting groups. In addition, suitable acyl groups were introduced for the protection of the lactam functions of guanine and uracil moieties.
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/17.20.8135