Conservation of the 5' end of the medRNA in New World species of Leishmania

The effectiveness of antisense oligonucleotides targeted against the spliced leader (SL) in trypanosomes has been documented by Verspieren at al. To evaluate a similar approach for leishmaniasis antisense therapy, we used a 12 base primer (Primer A) complementary to a conserved region of the SL to s...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Nucleic acids research 1989-10, Vol.17 (19), p.7999-7999
Hauptverfasser:	SCHOLLER, J. K, MCARDLE, S, REED, S, KANEMOTO, R
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Biological and medical sciences Fundamental and applied biological sciences. Psychology Genes. Genome Leishmania Leishmania - genetics Leishmania braziliensis Leishmania donovani Leishmania mexicana Molecular and cellular biology Molecular genetics Molecular Sequence Data Protein Sorting Signals RNA Precursors - genetics RNA, Messenger - genetics Sequence Homology, Nucleic Acid Species Specificity
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The effectiveness of antisense oligonucleotides targeted against the spliced leader (SL) in trypanosomes has been documented by Verspieren at al. To evaluate a similar approach for leishmaniasis antisense therapy, we used a 12 base primer (Primer A) complementary to a conserved region of the SL to sequence the 5' portion of the SL from the medRNA and the mature mRNAs of several leishmanial species. The first 15 nucleotides of the spliced leader was identical in the New World species of L. braziliensis quyanensis (Lbg), L. mexicana amazonensis (Lma), L. donovani chagasi (Ldc) and L. enriettii (Le).
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/17.19.7999