Analysis of 'color PCR' by automatic DNA sequencer

Recently Chehab and Kan developed a polymerase chain reaction (PCR) - based color complementation assay for the detection of DNA mutations. In this assay, competition between amplification primers conjugated to different fluorescent dyes is reflected by the color of the PCR products. We have develop...

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Veröffentlicht in:	Nucleic acids research 1989-09, Vol.17 (18), p.7538-7538
Hauptverfasser:	ZIMRAN, A, GLASS, C, SAMANTHA THORPE, V, BEUTLER, E
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Biological and medical sciences Fluorescent Dyes Fundamental and applied biological sciences. Psychology genes Glucosidases - genetics Glucosylceramidase - genetics Heterozygote Homozygote Humans Mutation Nucleic Acid Amplification Techniques polymerase chain reaction Polymerase Chain Reaction - methods
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Beschreibung
Zusammenfassung:	Recently Chehab and Kan developed a polymerase chain reaction (PCR) - based color complementation assay for the detection of DNA mutations. In this assay, competition between amplification primers conjugated to different fluorescent dyes is reflected by the color of the PCR products. We have developed a means for analyzing color-PCR products with an automated DNA sequencer and used this procedure to detect the point mutation 1226 of the glucocerebrosidase gene.
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/17.18.7538