A sensitive, bioluminescent-enhanced detection method for DNA dot-hybridization

The authors report here an alternative, sensitive, bioluminescent-enhanced detection method for DNA dot-hybridization. Immobilized DNA is hybridized with a biotinylated probe and incubated with a preformed streptavidin biotinylated alkaline phosphatase complex. Bound complex liberates D-luciferin fr...

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Veröffentlicht in:	Nucleic acids research 1988-02, Vol.16 (3), p.1213-1213
Hauptverfasser:	HAUBER, R, GEIGER, R
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences DNA - analysis Dna, deoxyribonucleoproteins Firefly Luciferin Fundamental and applied biological sciences. Psychology Luciferases Luminescent Measurements Methods Nucleic Acid Hybridization Nucleic acids
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Beschreibung
Zusammenfassung:	The authors report here an alternative, sensitive, bioluminescent-enhanced detection method for DNA dot-hybridization. Immobilized DNA is hybridized with a biotinylated probe and incubated with a preformed streptavidin biotinylated alkaline phosphatase complex. Bound complex liberates D-luciferin from the substrate D-luciferin-O-phosphate, and D-luciferin is oxidized by luciferase (Photinus pyralis ) under light emission. Emitted light exposes a sensitive photographic film (Kodac, Tri X-Pan). Thus specifically bound alkaline phosphatase complex can be visualized after development.
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/16.3.1213