Isolation and mapping of a polymorphic DNA sequence pMCT35.1 on chromosome 17p [D17S31]

A 2.4 kb TaqI fragment from cosmid MCT35 was subcloned into the AccI site of pUC18. MspI identifies a two allele polymorphism (M1:2.4 kb, M2:1.8 kb). RsaI also identifies a polymorphism, but this observation has not been verified by family studies. The frequency estimated from 109 unrelated Caucasia...

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Veröffentlicht in:	Nucleic acids research 1988-01, Vol.16 (2), p.783-783
Hauptverfasser:	CARLSON, M, NAKAMURA, Y, PAYSON, R, O'CONNELL, P, LEPPERT, M, LATHROP, G. M, LALOUEL, J.-M, WHITE, R
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Chromosome Mapping Chromosomes, Human, Pair 17 Cloning, Molecular Fundamental and applied biological sciences. Psychology Genes. Genome Humans Molecular and cellular biology Molecular genetics Polymorphism, Genetic Polymorphism, Restriction Fragment Length
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Beschreibung
Zusammenfassung:	A 2.4 kb TaqI fragment from cosmid MCT35 was subcloned into the AccI site of pUC18. MspI identifies a two allele polymorphism (M1:2.4 kb, M2:1.8 kb). RsaI also identifies a polymorphism, but this observation has not been verified by family studies. The frequency estimated from 109 unrelated Caucasians was MspI M1: 0.75 and M2:0.25. pMCT35.1 has been assigned to chromosome 17p multipoint linkage analysis with loci (D17S1, MYH2, D17Z1) known to span this region. Co-dominant segregation has been observed with the MspI RFLP in 55 three generation families.
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/16.2.783