Role of an upstream open reading frame in the translation of polycistronic mRNAs in plant cells
The influence of an upstream small open reading frame (URF) on the translation of two consecutive coding regions on an eukaryotic mRNA was studied. The cis effects of leader length, URF length, the sequences of the URF and neighboring regions, and the trans effects of the Cauliflower mosaic virus tr...
Gespeichert in:
| Veröffentlicht in: | Nucleic acids research 1992-08, Vol.20 (15), p.3851-3857 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3857 |
|---|---|
| container_issue | 15 |
| container_start_page | 3851 |
| container_title | Nucleic acids research |
| container_volume | 20 |
| creator | Futterer, J Zurich, Switzerland Hohn, T |
| description | The influence of an upstream small open reading frame (URF) on the translation of two consecutive coding regions on an eukaryotic mRNA was studied. The cis effects of leader length, URF length, the sequences of the URF and neighboring regions, and the trans effects of the Cauliflower mosaic virus transactivator (TAV) were analyzed. Translation efficiency of the immediate downstream open reading frame (ORF) decreased with increasing URF length. Short URFs did not drastically inhibit translation of immediate downstream ORFs but supported far downstream translation in the presence of TAV. in the latter case, the optimal URF length was 30 codons. |
| doi_str_mv | 10.1093/nar/20.15.3851 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_334058</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>19553466</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4211-362857dcc5003b6c0e1a9d9896b2744c9a77f083d236bd3d1c0e4286f376e8943</originalsourceid><addsrcrecordid>eNqFkc1v1DAQxS1EVZbClRvCh6q3bMffyYFDVdEW-oFUKEJcLK_jbAOJHewsov89jrLawonTjPR-bzQzD6FXBJYEKnbsTTymuRdLVgryBC0Ik7TglaRP0QIYiIIAL5-h5yl9ByCcCL6P9omAUipYIH0bOodDg43HmyGN0Zkeh8F5nLu69WvcRNM73Ho83js8RuNTZ8Y2-Mk0hO7BttkVfGtxf3tzkiZy6IwfsXVdl16gvcZ0yb3c1gN0d_bu8-lFcfXx_P3pyVVhOSWkyDuXQtXWCgC2khYcMVVdlZVcUcW5rYxSDZSspkyualaTTHBayoYp6cqKswP0dp47bFa9q63zedVOD7HtTXzQwbT6X8W393odfmnGOIgy-4-2_hh-blwadd-m6QLjXdgkrdj0OiL_C5JKCMblBC5n0MaQUnTNbhkCeopO5-g0zb3QU3TZ8PrvEx7xOausH251k6zpci4-_36HCS6BC5qxYsZyLu73Tjbxh5aKKaEvvn7TQG8uxZfzD_o6829mvjFBm3XMI-8-USAMiFJcUsn-AEI5uig</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19553466</pqid></control><display><type>article</type><title>Role of an upstream open reading frame in the translation of polycistronic mRNAs in plant cells</title><source>MEDLINE</source><source>Oxford University Press Journals Digital Archive Legacy</source><source>PubMed Central</source><creator>Futterer, J ; Zurich, Switzerland ; Hohn, T</creator><creatorcontrib>Futterer, J ; Zurich, Switzerland ; Hohn, T</creatorcontrib><description>The influence of an upstream small open reading frame (URF) on the translation of two consecutive coding regions on an eukaryotic mRNA was studied. The cis effects of leader length, URF length, the sequences of the URF and neighboring regions, and the trans effects of the Cauliflower mosaic virus transactivator (TAV) were analyzed. Translation efficiency of the immediate downstream open reading frame (ORF) decreased with increasing URF length. Short URFs did not drastically inhibit translation of immediate downstream ORFs but supported far downstream translation in the presence of TAV. in the latter case, the optimal URF length was 30 codons.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/20.15.3851</identifier><identifier>PMID: 1508670</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Base Sequence ; beta-glucuronidase ; Biological and medical sciences ; Brassicaceae ; Cauliflower mosaic virus ; chloramphenicol acetyltransferase ; cistrons ; Codon - genetics ; DNA-binding proteins ; Fundamental and applied biological sciences. Psychology ; gene expression ; genetic transformation ; messenger RNA ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Mosaic Viruses - genetics ; nucleotide sequences ; Open Reading Frames - genetics ; orychophragmus violaceus ; Plants - genetics ; Plants - microbiology ; Plasmids - genetics ; Protein Biosynthesis - genetics ; protoplasts ; Recombinant Fusion Proteins - genetics ; reporter genes ; RNA, Messenger - genetics ; RNA, Viral - genetics ; Trans-Activators - genetics ; translation ; Translation. Translation factors. Protein processing</subject><ispartof>Nucleic acids research, 1992-08, Vol.20 (15), p.3851-3857</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4211-362857dcc5003b6c0e1a9d9896b2744c9a77f083d236bd3d1c0e4286f376e8943</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC334058/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC334058/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5460452$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1508670$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Futterer, J</creatorcontrib><creatorcontrib>Zurich, Switzerland</creatorcontrib><creatorcontrib>Hohn, T</creatorcontrib><title>Role of an upstream open reading frame in the translation of polycistronic mRNAs in plant cells</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>The influence of an upstream small open reading frame (URF) on the translation of two consecutive coding regions on an eukaryotic mRNA was studied. The cis effects of leader length, URF length, the sequences of the URF and neighboring regions, and the trans effects of the Cauliflower mosaic virus transactivator (TAV) were analyzed. Translation efficiency of the immediate downstream open reading frame (ORF) decreased with increasing URF length. Short URFs did not drastically inhibit translation of immediate downstream ORFs but supported far downstream translation in the presence of TAV. in the latter case, the optimal URF length was 30 codons.</description><subject>Base Sequence</subject><subject>beta-glucuronidase</subject><subject>Biological and medical sciences</subject><subject>Brassicaceae</subject><subject>Cauliflower mosaic virus</subject><subject>chloramphenicol acetyltransferase</subject><subject>cistrons</subject><subject>Codon - genetics</subject><subject>DNA-binding proteins</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>genetic transformation</subject><subject>messenger RNA</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Mosaic Viruses - genetics</subject><subject>nucleotide sequences</subject><subject>Open Reading Frames - genetics</subject><subject>orychophragmus violaceus</subject><subject>Plants - genetics</subject><subject>Plants - microbiology</subject><subject>Plasmids - genetics</subject><subject>Protein Biosynthesis - genetics</subject><subject>protoplasts</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>reporter genes</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Viral - genetics</subject><subject>Trans-Activators - genetics</subject><subject>translation</subject><subject>Translation. Translation factors. Protein processing</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS1EVZbClRvCh6q3bMffyYFDVdEW-oFUKEJcLK_jbAOJHewsov89jrLawonTjPR-bzQzD6FXBJYEKnbsTTymuRdLVgryBC0Ik7TglaRP0QIYiIIAL5-h5yl9ByCcCL6P9omAUipYIH0bOodDg43HmyGN0Zkeh8F5nLu69WvcRNM73Ho83js8RuNTZ8Y2-Mk0hO7BttkVfGtxf3tzkiZy6IwfsXVdl16gvcZ0yb3c1gN0d_bu8-lFcfXx_P3pyVVhOSWkyDuXQtXWCgC2khYcMVVdlZVcUcW5rYxSDZSspkyualaTTHBayoYp6cqKswP0dp47bFa9q63zedVOD7HtTXzQwbT6X8W393odfmnGOIgy-4-2_hh-blwadd-m6QLjXdgkrdj0OiL_C5JKCMblBC5n0MaQUnTNbhkCeopO5-g0zb3QU3TZ8PrvEx7xOausH251k6zpci4-_36HCS6BC5qxYsZyLu73Tjbxh5aKKaEvvn7TQG8uxZfzD_o6829mvjFBm3XMI-8-USAMiFJcUsn-AEI5uig</recordid><startdate>19920811</startdate><enddate>19920811</enddate><creator>Futterer, J</creator><creator>Zurich, Switzerland</creator><creator>Hohn, T</creator><general>Oxford University Press</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19920811</creationdate><title>Role of an upstream open reading frame in the translation of polycistronic mRNAs in plant cells</title><author>Futterer, J ; Zurich, Switzerland ; Hohn, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4211-362857dcc5003b6c0e1a9d9896b2744c9a77f083d236bd3d1c0e4286f376e8943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Base Sequence</topic><topic>beta-glucuronidase</topic><topic>Biological and medical sciences</topic><topic>Brassicaceae</topic><topic>Cauliflower mosaic virus</topic><topic>chloramphenicol acetyltransferase</topic><topic>cistrons</topic><topic>Codon - genetics</topic><topic>DNA-binding proteins</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>genetic transformation</topic><topic>messenger RNA</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Mosaic Viruses - genetics</topic><topic>nucleotide sequences</topic><topic>Open Reading Frames - genetics</topic><topic>orychophragmus violaceus</topic><topic>Plants - genetics</topic><topic>Plants - microbiology</topic><topic>Plasmids - genetics</topic><topic>Protein Biosynthesis - genetics</topic><topic>protoplasts</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>reporter genes</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Viral - genetics</topic><topic>Trans-Activators - genetics</topic><topic>translation</topic><topic>Translation. Translation factors. Protein processing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Futterer, J</creatorcontrib><creatorcontrib>Zurich, Switzerland</creatorcontrib><creatorcontrib>Hohn, T</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Futterer, J</au><au>Zurich, Switzerland</au><au>Hohn, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of an upstream open reading frame in the translation of polycistronic mRNAs in plant cells</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1992-08-11</date><risdate>1992</risdate><volume>20</volume><issue>15</issue><spage>3851</spage><epage>3857</epage><pages>3851-3857</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>The influence of an upstream small open reading frame (URF) on the translation of two consecutive coding regions on an eukaryotic mRNA was studied. The cis effects of leader length, URF length, the sequences of the URF and neighboring regions, and the trans effects of the Cauliflower mosaic virus transactivator (TAV) were analyzed. Translation efficiency of the immediate downstream open reading frame (ORF) decreased with increasing URF length. Short URFs did not drastically inhibit translation of immediate downstream ORFs but supported far downstream translation in the presence of TAV. in the latter case, the optimal URF length was 30 codons.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>1508670</pmid><doi>10.1093/nar/20.15.3851</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1992-08, Vol.20 (15), p.3851-3857 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_334058 |
| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy; PubMed Central |
| subjects | Base Sequence beta-glucuronidase Biological and medical sciences Brassicaceae Cauliflower mosaic virus chloramphenicol acetyltransferase cistrons Codon - genetics DNA-binding proteins Fundamental and applied biological sciences. Psychology gene expression genetic transformation messenger RNA Molecular and cellular biology Molecular genetics Molecular Sequence Data Mosaic Viruses - genetics nucleotide sequences Open Reading Frames - genetics orychophragmus violaceus Plants - genetics Plants - microbiology Plasmids - genetics Protein Biosynthesis - genetics protoplasts Recombinant Fusion Proteins - genetics reporter genes RNA, Messenger - genetics RNA, Viral - genetics Trans-Activators - genetics translation Translation. Translation factors. Protein processing |
| title | Role of an upstream open reading frame in the translation of polycistronic mRNAs in plant cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T17%3A49%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Role%20of%20an%20upstream%20open%20reading%20frame%20in%20the%20translation%20of%20polycistronic%20mRNAs%20in%20plant%20cells&rft.jtitle=Nucleic%20acids%20research&rft.au=Futterer,%20J&rft.date=1992-08-11&rft.volume=20&rft.issue=15&rft.spage=3851&rft.epage=3857&rft.pages=3851-3857&rft.issn=0305-1048&rft.eissn=1362-4962&rft.coden=NARHAD&rft_id=info:doi/10.1093/nar/20.15.3851&rft_dat=%3Cproquest_pubme%3E19553466%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19553466&rft_id=info:pmid/1508670&rfr_iscdi=true |