Role of an upstream open reading frame in the translation of polycistronic mRNAs in plant cells

The influence of an upstream small open reading frame (URF) on the translation of two consecutive coding regions on an eukaryotic mRNA was studied. The cis effects of leader length, URF length, the sequences of the URF and neighboring regions, and the trans effects of the Cauliflower mosaic virus tr...

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Veröffentlicht in:	Nucleic acids research 1992-08, Vol.20 (15), p.3851-3857
Hauptverfasser:	Futterer, J, Zurich, Switzerland, Hohn, T
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence beta-glucuronidase Biological and medical sciences Brassicaceae Cauliflower mosaic virus chloramphenicol acetyltransferase cistrons Codon - genetics DNA-binding proteins Fundamental and applied biological sciences. Psychology gene expression genetic transformation messenger RNA Molecular and cellular biology Molecular genetics Molecular Sequence Data Mosaic Viruses - genetics nucleotide sequences Open Reading Frames - genetics orychophragmus violaceus Plants - genetics Plants - microbiology Plasmids - genetics Protein Biosynthesis - genetics protoplasts Recombinant Fusion Proteins - genetics reporter genes RNA, Messenger - genetics RNA, Viral - genetics Trans-Activators - genetics translation Translation. Translation factors. Protein processing
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Zusammenfassung:	The influence of an upstream small open reading frame (URF) on the translation of two consecutive coding regions on an eukaryotic mRNA was studied. The cis effects of leader length, URF length, the sequences of the URF and neighboring regions, and the trans effects of the Cauliflower mosaic virus transactivator (TAV) were analyzed. Translation efficiency of the immediate downstream open reading frame (ORF) decreased with increasing URF length. Short URFs did not drastically inhibit translation of immediate downstream ORFs but supported far downstream translation in the presence of TAV. in the latter case, the optimal URF length was 30 codons.
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/20.15.3851