Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products

Although the polymerase chain reaction (PCR) can be used to produce a large amount of a specific DNA from a complex source, cloning the PCR products has not proven to be straightforward. This template-independent activity of Taq polymerase can be exploited to create a cloning scheme which has the ef...

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Veröffentlicht in:	Nucleic acids research 1991-03, Vol.19 (5), p.1154-1154
Hauptverfasser:	MARCHUK, D, DRUMM, M, SAULINO, A, COLLINS, F. S
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Cloning, Molecular - methods Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Genetic Vectors Methods. Procedures. Technologies Molecular cloning Polymerase Chain Reaction
Online-Zugang:	Volltext
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