Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products

Although the polymerase chain reaction (PCR) can be used to produce a large amount of a specific DNA from a complex source, cloning the PCR products has not proven to be straightforward. This template-independent activity of Taq polymerase can be exploited to create a cloning scheme which has the efficiency of sticky end cloning, but requires no additional enzymatic modification of the PCR product.