Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products
Although the polymerase chain reaction (PCR) can be used to produce a large amount of a specific DNA from a complex source, cloning the PCR products has not proven to be straightforward. This template-independent activity of Taq polymerase can be exploited to create a cloning scheme which has the ef...
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| Veröffentlicht in: | Nucleic acids research 1991-03, Vol.19 (5), p.1154-1154 |
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| container_issue | 5 |
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| container_title | Nucleic acids research |
| container_volume | 19 |
| creator | MARCHUK, D DRUMM, M SAULINO, A COLLINS, F. S |
| description | Although the polymerase chain reaction (PCR) can be used to produce a large amount of a specific DNA from a complex source, cloning the PCR products has not proven to be straightforward. This template-independent activity of Taq polymerase can be exploited to create a cloning scheme which has the efficiency of sticky end cloning, but requires no additional enzymatic modification of the PCR product. |
| doi_str_mv | 10.1093/nar/19.5.1154 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1991-03, Vol.19 (5), p.1154-1154 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_333800 |
| source | MEDLINE; PubMed Central; Oxford University Press Journals Digital Archive Legacy |
| subjects | Biological and medical sciences Biotechnology Cloning, Molecular - methods Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Genetic Vectors Methods. Procedures. Technologies Molecular cloning Polymerase Chain Reaction |
| title | Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products |
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