Modified deoxyoligonucleotides stable to exonuclease degradation in serum

Unmodified deoxyoligonucleotides are rapidly degraded in serum-containing medium. Utilizing internally labelled deoxyoligonucleotides the deoxyribonuclease profile for fetal calf serum and human serum was determined. It was found that the predominate nuclease activity in both systems was 3' exo...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Nucleic acids research 1991-02, Vol.19 (4), p.747-750
Hauptverfasser:	SHAW, J.-P, KENT, K, BIRD, J, FISHBACK, J, FROEHLER, B
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Base Sequence Biological and medical sciences Blood Chromatography, High Pressure Liquid Dna, deoxyribonucleoproteins Electrophoresis, Polyacrylamide Gel Exonucleases - chemistry Fundamental and applied biological sciences. Psychology Hot Temperature Humans Molecular Sequence Data Nucleic Acid Denaturation Nucleic acids Oligonucleotides - chemistry Oxygen - chemistry Tumor Cells, Cultured
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Unmodified deoxyoligonucleotides are rapidly degraded in serum-containing medium. Utilizing internally labelled deoxyoligonucleotides the deoxyribonuclease profile for fetal calf serum and human serum was determined. It was found that the predominate nuclease activity in both systems was 3' exonuclease. Deoxyoligonucleotides are protected from exonuclease degradation in sera and cell media by simple terminal modifications that maintain high binding activity for the complementary DNA sequence.
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/19.4.747