Quantitative Proteomics Reveals the Basis for the Biochemical Specificity of the Cell-Cycle Machinery

Cyclin-dependent kinases comprise the conserved machinery that drives progress through the cell cycle, but how they do this in mammalian cells is still unclear. To identify the mechanisms by which cyclin-cdks control the cell cycle, we performed a time-resolved analysis of the in vivo interactors of cyclins E1, A2, and B1 by quantitative mass spectrometry. This global analysis of context-dependent protein interactions reveals the temporal dynamics of cyclin function in which networks of cyclin-cdk interactions vary according to the type of cyclin and cell-cycle stage. Our results explain the temporal specificity of the cell-cycle machinery, thereby providing a biochemical mechanism for the genetic requirement for multiple cyclins in vivo and reveal how the actions of specific cyclins are coordinated to control the cell cycle. Furthermore, we identify key substrates (Wee1 and c15orf42/Sld3) that reveal how cyclin A is able to promote both DNA replication and mitosis. ► Quantitative proteomic strategy reveals dynamics of cell-cycle protein interactions ► Cyclins confer biochemical specificity to cyclin/cdk interaction networks ► Cyclin A phosphorylates Sld3/c15orf42 and Wee1 to promote S phase and mitosis ► Cyclins A and B interact sequentially with proteins in G2 phase and mitosis