E. coli dam activity in hepes buffer asks for a new unit definition

The E. coli DNA-adenine-methyltransferase (Dam) is an enzyme that exerts multiple functions in the cell. Formerly Tris/Cl- or Phosphate-buffer have been used for purification and assays of the protein. We found that Hepes/KOH-buffer renders Dam about 3 times more active, than the conventional buffer...

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Veröffentlicht in:	Nucleic acids research 1990-12, Vol.18 (23), p.7189-7189
Hauptverfasser:	HUÊLSMANN, K.-H, BERGERAT-COULAUD, A, HAHN, U
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cattle DNA - metabolism Enzymes and enzyme inhibitors Escherichia coli Escherichia coli - enzymology Fundamental and applied biological sciences. Psychology HEPES Kinetics Site-Specific DNA-Methyltransferase (Adenine-Specific) - metabolism Transferases
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Zusammenfassung:	The E. coli DNA-adenine-methyltransferase (Dam) is an enzyme that exerts multiple functions in the cell. Formerly Tris/Cl- or Phosphate-buffer have been used for purification and assays of the protein. We found that Hepes/KOH-buffer renders Dam about 3 times more active, than the conventional buffer, which has been used for the unit definition.
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/18.23.7189