Sequential determination system for anionic detergents by complexation with methylene blue by dual high-speed counter-current chromatography

A new dual high-speed counter-current chromatographic system using organic extraction phase and aqueous mobile phase containing methylene blue was applied to the analysis of anionic-type detergents. After selecting appropriate conditions such as flow rate of each mobile phase, sample volume, etc., the new system was successfully applied to the analysis of anionic detergent in river water. As all the analytical procedure can be made in a closed system, the method has no health hazard. The present method is simple, safe, and highly sensitive, and can be applied for sequential determination of multiple samples in a short analysis time.