Determination of RET Sequence Variation in an MEN2 Unaffected Cohort Using Multiple-Sample Pooling and Next-Generation Sequencing

Determination of RET Sequence Variation in an MEN2 Unaffected Cohort Using Multiple-Sample Pooling and Next-Generation Sequencing

Multisample, nonindexed pooling combined with next-generation sequencing (NGS) was used to discover RET proto-oncogene sequence variation within a cohort known to be unaffected by multiple endocrine neoplasia type 2 (MEN2). DNA samples (113 Caucasians, 23 persons of other ethnicities) were amplified...

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Veröffentlicht in:Journal of Thyroid Research 2012-01, Vol.2012 (2012), p.55-63
Hauptverfasser: Margraf, R. L., Durtschi, J. D., Stephens, J. E., Perez, M., Voelkerding, K. V.
Format: Artikel
Sprache:eng
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Zusammenfassung:Multisample, nonindexed pooling combined with next-generation sequencing (NGS) was used to discover RET proto-oncogene sequence variation within a cohort known to be unaffected by multiple endocrine neoplasia type 2 (MEN2). DNA samples (113 Caucasians, 23 persons of other ethnicities) were amplified for RET intron 9 to intron 16 and then divided into 5 pools of
ISSN:2042-0072
2090-8067
2042-0072
DOI:10.1155/2012/318232