An improved method for directly sequencing PCR amplified material using dimethyl sulphoxide

The use of the dideoxy chain termination procedure to sequence double stranded DNA templates is subject to problems related to the template strands reannealing. The author found that when using Sequenase super(Tm) (USB), the inclusion of dimethyl sulphoxide (DMSO) during the sequencing reactions hel...

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Veröffentlicht in:	Nucleic acids research 1989-02, Vol.17 (3), p.1266-1266
1. Verfasser:	WINSHIP, P. R
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Dimethyl Sulfoxide Diverse techniques DNA DNA - isolation & purification DNA-Directed DNA Polymerase Factor IX - genetics Fundamental and applied biological sciences. Psychology Gene Amplification Humans Molecular and cellular biology nucleotide sequence
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Zusammenfassung:	The use of the dideoxy chain termination procedure to sequence double stranded DNA templates is subject to problems related to the template strands reannealing. The author found that when using Sequenase super(Tm) (USB), the inclusion of dimethyl sulphoxide (DMSO) during the sequencing reactions helps to overcome these problems.
ISSN:	0305-1048 1362-4962