Two polymorphic TaqI sites at the human NGFR locus (17q12→17q22)

The cosmid clone C3 contains human NGFR sequences cloned in the JB8 vector. TaqI (TCGA) identifies two site dimorphisms. Constant bands are observed at 6.5 kb, 3.2 kb, 2.3 kb, 2.0 kb, 1.15 kb and 0.85 kb. Combined heterozygosity is 55%. The NGFR locus has been assigned to 17q12->17q22 with somati...

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Veröffentlicht in:	Nucleic acids research 1989-01, Vol.17 (2), p.824-824
Hauptverfasser:	WRIGHT, E. C, FAIN, P. R, BARKER, D. F, CHAO, M. V
Format:	Artikel
Sprache:	eng
Schlagworte:	550200 - Biochemistry BASIC BIOLOGICAL SCIENCES Biological and medical sciences Chromosome Mapping CHROMOSOMES Chromosomes, Human, Pair 17 CLONING Deoxyribonucleases, Type II Site-Specific DNA DNA HYBRIDIZATION DNA SEQUENCING DNA-CLONING Fundamental and applied biological sciences. Psychology Genes. Genome GENETIC MAPPING HETEROCHROMOSOMES HUMAN POPULATIONS Humans HYBRIDIZATION MAPPING Molecular and cellular biology Molecular genetics Nerve Growth Factors - metabolism NUCLEIC ACIDS ORGANIC COMPOUNDS Polymorphism, Genetic Polymorphism, Restriction Fragment Length POPULATIONS Receptors, Cell Surface - genetics Receptors, Nerve Growth Factor RECOMBINANT DNA RFLPS STRUCTURAL CHEMICAL ANALYSIS
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Zusammenfassung:	The cosmid clone C3 contains human NGFR sequences cloned in the JB8 vector. TaqI (TCGA) identifies two site dimorphisms. Constant bands are observed at 6.5 kb, 3.2 kb, 2.3 kb, 2.0 kb, 1.15 kb and 0.85 kb. Combined heterozygosity is 55%. The NGFR locus has been assigned to 17q12->17q22 with somatic cell hybrid analysis and in situ hybridization. The TaqI site polymorphisms show tight linkage to HOX2 and other 17q markers and loose linkage to NFl. No significant deviation from Hardy-Weinberg equilibrium has been observed for either system. Proper Mendelian segregation has been observed in 45 offspring of parents segregating system A and 31 offspring of parents segregating B alleles.
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/17.2.824