Nucleotide sequence of the Fbp gene from Neisseria meningitidis

The gene that encodes the major iron regulated protein (Fbp) of Neisseria gonorrhoeae has been cloned and sequenced. Results of N-terminal amino acid sequence analysis and reactivity with monoclonal and polyclonal antibodies suggested that a similar protein was present in the other pathogenic species of Neisseria, Neisseria meningitidis . Sequence analysis of the Fbp gene has confirmed this homology. We have cloned the Fbp gene of N. meningitidis (strain 80084313, serogroup A) on two genome TaqI fragments in pUC 13 using probes from the N. Gonorrhoeae Fbp gene. Sequence data from the N. meningitidis Fbp gene was compared to that of N. gonorrhoeae , (strain F62) from the start of the leader peptide through 141 bases of the 3' untranslated region.