Cationic lipid-mediated co-transfection of insect cells

We report a more effective and convenient method that uses the cationic lipid N(1,1-(2,3-dioleyloxy)propoyl)-N,N,N,-trimethylammonium chloride, or DOTMA, for cotransfections of Sf-9 cells and used 96-well microtiter plates to identify and purify recombinant baculoviruses. The co-transfection method...

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Veröffentlicht in:	Nucleic acids research 1990-07, Vol.18 (13), p.4033-4033
Hauptverfasser:	GROEBE, D. R, CHUNG, A. E, CHIEN HO
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Biotechnology Cell Line Cloning, Molecular Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Hemoglobins - genetics Insecta Lepidoptera Methods. Procedures. Technologies Noctuidae Nucleic Acid Hybridization Quaternary Ammonium Compounds Spodoptera frugiperda Transfection
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Beschreibung
Zusammenfassung:	We report a more effective and convenient method that uses the cationic lipid N(1,1-(2,3-dioleyloxy)propoyl)-N,N,N,-trimethylammonium chloride, or DOTMA, for cotransfections of Sf-9 cells and used 96-well microtiter plates to identify and purify recombinant baculoviruses. The co-transfection method is not pH sensitive and may be used with vector DNA from mini-lysate protocols. DOTMA is sold by Gibco/BRL under the trade name Lipofectin super(TM).
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/18.13.4033