Spatial imaging of luciferase gene expression in transgenic fish

Gene expression is regulated in a temporal and tissue-specific manner during embryogenesis. The luciferase gene is used as a visible indicator for gene expression. We microinjected pRSV DNA containing firefly luciferase gene into nuclei of Medaka (Oryzias latipes ) oocytes.

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Bibliographische Detailangaben
Veröffentlicht in:	Nucleic acids research 1990-02, Vol.18 (4), p.1072-1072
Hauptverfasser:	TAMIYA, E, SUGIYAMA, T, MASAKI, K, HIROSE, A, OKOSHI, T, KARUBE, I
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Biotechnology Coleoptera - enzymology Coleoptera - genetics Cyprinodontiformes - genetics Embryo, Nonmammalian - cytology Embryo, Nonmammalian - enzymology Female Fertilization Fundamental and applied biological sciences. Psychology Gene Expression Genes Genetic engineering Genetic technics Luciferases - genetics Luminescent Measurements Methods. Procedures. Technologies Oryzias - genetics Oryzias latipes Transgenic animals Transgenic animals and transgenic plants
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Beschreibung
Zusammenfassung:	Gene expression is regulated in a temporal and tissue-specific manner during embryogenesis. The luciferase gene is used as a visible indicator for gene expression. We microinjected pRSV DNA containing firefly luciferase gene into nuclei of Medaka (Oryzias latipes ) oocytes.
ISSN:	0305-1048 1362-4962
DOI:	10.1093/nar/18.4.1072