cDNA sequence of the env gene of a pathogenic equine infectious anemia lentivirus variant

We report the env gene nucleotide sequence from a biologically cloned, virulent variant of equine infectious anemia virus (EIA V-WSU5). Dideoxynucleotide chain-termination sequencing of both strands of 2 overlapping cDNA clones isolated from cDNA libraries using cross-hybridizing EIA V-prototype env gene probes was done. EIA V-WSU5 env gene was the same length, 2,580 base pairs, as the published prototype EIA V env gene coding gp90 and gp45. There were 13 nucleotide substitutions between the env gene of EIA V-WSU5 and prototype virus; 6 resulted in amino acid changes.