General method for direct cloning of DNA fragments generated by the polymerase chain reaction
A general method is described for direct cloning of DNA fragments generated by primed enzymatic amplification that is based on digestibility of the cloning vector with XcmI restriction endonuclease to yield a unit linear molecule with 3' unpaired deoxythymidine residues at both ends. Such vecto...
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| Veröffentlicht in: | Nucleic acids research 1991-08, Vol.19 (16), p.4560-4560 |
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| container_end_page | 4560 |
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| container_issue | 16 |
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| container_title | Nucleic acids research |
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| creator | KOVALIC, D JIN-HWAN KWAK WEISBLUM, B |
| description | A general method is described for direct cloning of DNA fragments generated by primed enzymatic amplification that is based on digestibility of the cloning vector with XcmI restriction endonuclease to yield a unit linear molecule with 3' unpaired deoxythymidine residues at both ends. Such vectors are capable of efficient ligation with products that are obtained by primed enzymatic amplification with Taq DNA polymerase bearing 3' unpaired deoxyadenosine residues at both ends. |
| doi_str_mv | 10.1093/nar/19.16.4560 |
| format | Article |
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Such vectors are capable of efficient ligation with products that are obtained by primed enzymatic amplification with Taq DNA polymerase bearing 3' unpaired deoxyadenosine residues at both ends.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/19.16.4560</identifier><identifier>PMID: 1886782</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Base Sequence ; Biological and medical sciences ; Biotechnology ; Cloning, Molecular - methods ; DNA - metabolism ; DNA-Directed DNA Polymerase - metabolism ; Endodeoxyribonucleases - metabolism ; Fundamental and applied biological sciences. Psychology ; genes ; Genetic engineering ; Genetic technics ; Genetic Vectors - genetics ; Methods. Procedures. 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Such vectors are capable of efficient ligation with products that are obtained by primed enzymatic amplification with Taq DNA polymerase bearing 3' unpaired deoxyadenosine residues at both ends.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cloning, Molecular - methods</subject><subject>DNA - metabolism</subject><subject>DNA-Directed DNA Polymerase - metabolism</subject><subject>Endodeoxyribonucleases - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetic Vectors - genetics</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular cloning</subject><subject>Molecular Sequence Data</subject><subject>plasmids</subject><subject>Polymerase Chain Reaction</subject><subject>Taq Polymerase</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtvEzEUhS0EKqGwZYfkBWI3qd8eL7qoUihIFWzaJbI8njuJ0Yyd2pNK-fc4JCqw6upK93znPnQQek_JkhLDL6LLF9QsqVoKqcgLtKBcsUYYxV6iBeFENpSI9jV6U8ovQqigUpyhM9q2SrdsgX7eQITsRjzBvEk9HlLGfcjgZ-zHFENc4zTg6-9XeMhuPUGcC17_sczQ426P5w3gbRr3U20VwH7jQsQZnJ9Dim_Rq8GNBd6d6jm6__L5bvW1uf1x8211ddt4KejcSOGZk6ZXQAD8ICTpNNcdtF3rTWukBKY6MwgxaMlZ31PGHOscZ0x4R5zi5-jyOHe76ybofT2z_mS3OUwu721ywf6vxLCx6_RoOWuV1NX_6eTP6WEHZbZTKB7G0UVIu2I1I9poLZ4FqeGKVvR5UBHCuTEVXB5Bn1MpGYanqymxh4RtTbjOrQ57SLgaPvz761_8GGnVP550V7wba2zRh_KEycNiIflv2Kqv-g</recordid><startdate>19910825</startdate><enddate>19910825</enddate><creator>KOVALIC, D</creator><creator>JIN-HWAN KWAK</creator><creator>WEISBLUM, B</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T3</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19910825</creationdate><title>General method for direct cloning of DNA fragments generated by the polymerase chain reaction</title><author>KOVALIC, D ; JIN-HWAN KWAK ; WEISBLUM, B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c541t-54c2a59d6e0eecf450b737be8b8c98955e26b9f44f7532dd122a2ba3224ca0a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cloning, Molecular - methods</topic><topic>DNA - metabolism</topic><topic>DNA-Directed DNA Polymerase - metabolism</topic><topic>Endodeoxyribonucleases - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetic Vectors - genetics</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular cloning</topic><topic>Molecular Sequence Data</topic><topic>plasmids</topic><topic>Polymerase Chain Reaction</topic><topic>Taq Polymerase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KOVALIC, D</creatorcontrib><creatorcontrib>JIN-HWAN KWAK</creatorcontrib><creatorcontrib>WEISBLUM, B</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Human Genome Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KOVALIC, D</au><au>JIN-HWAN KWAK</au><au>WEISBLUM, B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>General method for direct cloning of DNA fragments generated by the polymerase chain reaction</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1991-08-25</date><risdate>1991</risdate><volume>19</volume><issue>16</issue><spage>4560</spage><epage>4560</epage><pages>4560-4560</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>A general method is described for direct cloning of DNA fragments generated by primed enzymatic amplification that is based on digestibility of the cloning vector with XcmI restriction endonuclease to yield a unit linear molecule with 3' unpaired deoxythymidine residues at both ends. Such vectors are capable of efficient ligation with products that are obtained by primed enzymatic amplification with Taq DNA polymerase bearing 3' unpaired deoxyadenosine residues at both ends.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>1886782</pmid><doi>10.1093/nar/19.16.4560</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Nucleic acids research, 1991-08, Vol.19 (16), p.4560-4560 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_328657 |
| source | Oxford University Press Journals Digital Archive legacy; MEDLINE; PubMed Central |
| subjects | Base Sequence Biological and medical sciences Biotechnology Cloning, Molecular - methods DNA - metabolism DNA-Directed DNA Polymerase - metabolism Endodeoxyribonucleases - metabolism Fundamental and applied biological sciences. Psychology genes Genetic engineering Genetic technics Genetic Vectors - genetics Methods. Procedures. Technologies Molecular cloning Molecular Sequence Data plasmids Polymerase Chain Reaction Taq Polymerase |
| title | General method for direct cloning of DNA fragments generated by the polymerase chain reaction |
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